Target nomination resources

Instructions for preparing a target nomination proposal
Template community outreach target nomination form
PSI community outreach target nomination policy statement
PSI Structural Biology Knowledgebase community outreach target nomination portal


NESG community outreach structure gallery

NESG community outreach structures

NESG community outreach highlights
Some 502 Community Outreach Targets representing both nominated targets (upper panel) and collaborations on solved NESG targets are included in the current NESG Target List. Some sixty-seven (67) structures (for 58 targets) from the Community Outreach target list have been deposited in the PDB (lower panel) in PSI-1 and PSI-2 representing about 9% of the total output of the NESG.



NESG community outreach target highlights


Bacterial tRNA Isopentenyl adenosyl transferase A (MiaA)

X-Ray S. Chimnaronk, Mahidol University, Thailand,
I. Tanaka, Hokkaido University, Japan M. Atta
and M. Fontecave, Institut de Recherches en
Technologie et Sciences pour le Vivant, France
NESG vignette structures: 3EXA 2QGN 3D3Q
Chimnaronk S, Forouhar F, Sakai J, Yao M, Tron CM, Atta M, Fontecave M, Hunt JF, Tanaka I. Snapshots of Dynamics in Synthesizing N6-isopentenyladenosine at tRNA Anticodon. Biochemistry. 2009 Jun 16;48(23):5057-65.


Salicylic Acid Binding Protein (SABP2) is Methylsalycitate Esterase essential for plant defense reaction
X-Ray Dan Klessig, Cornell University;
Boyce Thompson Institute for Plant Research
PSI-SKB highlight NESG vignette structures: 1Y7H 1Y7I 1XKL
Forouhar F, Yang Y, Kumar D, Chen Y, Fridman E, Park SW, Chiang Y, Acton TB, Montelione GT, Pichersky E, Klessig DF, Tong L (2005) Structural and biochemical studies identify tobacco SABP2 as a methyl salicylate esterase and implicate it in plant innate immunity. Proceedings of the National Academy of Sciences of the United States of America, 102(5), 1773-8.
Forouhar F, Kuzin A, Seetharaman J, Lee I, Zhou W, Abashidze M, Chen Y, Yong W, Janjua H, Fang Y, Wang D, Cunningham K, Xiao R, Acton TB, Pichersky E, Klessig DF, Porter CW, Montelione GT, Tong L (2007) Functional insights from structural genomics. Journal of Structural and Functional Genomics, 8, 37-44.
Zhao N, Guan J, Forouhar F, Tschaplinski TJ, Cheng ZM, Tong L, Chen F (2009) Two poplar methyl salicylate esterases display comparable biochemical properties but divergent expression patterns. Phytochemistry, 70, 37-44.


Influenza A virus NS1 Effector Domain : Human CPSF30 complex
X-Ray Robert Krug, University of Texas at Austin PSI-SKB highlight NESG vignette structure: 2RHK
Das K, Ma LC, Xiao R, Radvansky B, Aramini J, Zhao L, Marklund J, Kuo RL, Twu KY, Arnold E, Krug RM, Montelione GT (2008) Structural basis for suppression of a host antiviral response by influenza A virus. Proceedings of the National Academy of Sciences of the United States of America, 105, 13093-8.


Influenza A virus NS1 dsRNA-binding domain
X-Ray Robert Krug, University of Texas at Austin structure: 2RHK
Yin C, Khan JA, Swapna GV, Ertekin A, Krug RM, Tong L, Montelione GT (2007) Conserved surface features form the double-stranded RNA binding site of non-structural protein 1 (NS1) from influenza A and B viruses. Journal of Biological Chemistry, 282(28), 20584-92.


Polyamine Synthesis Enzymes
X-Ray Carl Porter, Roswell Park Cancer Institute NESG vignette structures:
1RTY 1SQS 1TM0 2HD3
2NV4 2OYS 1Z6P
Forouhar F, Lee IS, Vujcic J, Vujcic S, Shen J, Vorobiev SM, Xiao R, Acton TB, Montelione GT, Porter CW, Tong L (2005) Structural and functional evidence for Bacillus subtilis PaiA as a novel N1-spermidine/spermine acetyltransferase. Journal of Biological Chemistry, 280(48), 40328-36.
Forouhar F, Kuzin A, Seetharaman J, Lee I, Zhou W, Abashidze M, Chen Y, Yong W, Janjua H, Fang Y, Wang D, Cunningham K, Xiao R, Acton TB, Pichersky E, Klessig DF, Porter CW, Montelione GT, Tong L (2007) Functional insights from structural genomics. Journal of Structural and Functional Genomics, 8, 37-44.


Herpes virus proteins
X-Ray Ren Sun, University of California Los Angeles
Hongyu Deng, Institute of Biophysics, Chinese Academy of Sciences, Beijing
NESG vignette structure: 2OA5
Benach J, Wang L, Chen Y, Ho CK, Lee S, Seetharaman J, Xiao R, Acton TB, Montelione GT, Deng H, Sun R, Tong L (2007) Structural and functional studies of the abundant tegument protein ORF52 from murine gammaherpesvirus 68. Journal of Biological Chemistry, 282, 31534-41.


Tryptophan deoxygenase
X-Ray Stephen Chapman, University of Edinburgh NESG vignette structures: 2NW7 2NWB 2NW8 2NW9
Forouhar F, Anderson JL, Mowat CG, Vorobiev SM, Hussain A, Abashidze M, Bruckmann C, Thackray SJ, Seetharaman J, Tucker T, Xiao R, Ma LC, Zhao L, Acton TB, Montelione GT, Chapman SK, Tong L (2007) Molecular insights into substrate recognition and catalysis by tryptophan 2,3-dioxygenase. Proceedings of the National Academy of Sciences of the United States of America, 104(2), 473-8.


Antibacterial E coli Microcin J25 - A RNA Polymerase Inhibitor
NMR Richard Ebright, Rutgers University structure: 1PP5
Bayro MJ, Mukhopadhyay J, Swapna GV, Huang JY, Ma LC, Sineva E, Dawson PE, Montelione GT, Ebright RH (2003) Structure of antibacterial peptide microcin J25: a 21-residue lariat protoknot. Journal of the American Chemical Society, 125(41), 12382-3.


The Mammallian Muscle Tropomyosin Junction
NMR Sarah Hitchcock-DeGregori,
Robert Wood Johnson Medical School, UMDNJ
structures: 2G9J 1MV4 1IHQ
Greenfield NJ, Huang YJ, Swapna GV, Bhattacharya A, Rapp B, Singh A, Montelione GT, Hitchcock-DeGregori SE (2006) Solution NMR structure of the junction between tropomyosin molecules: implications for actin binding and regulation. Journal of Molecular Biology, 364(1), 80-96.
Greenfield NJ, Swapna GV, Huang Y, Palm T, Graboski S, Montelione GT, Hitchcock-DeGregori SE (2003) The structure of the carboxyl terminus of striated alpha-tropomyosin in solution reveals an unusual parallel arrangement of interacting alpha-helices. Biochemistry, 42(3), 614-9.
Greenfield NJ, Huang YJ, Palm T, Swapna GV, Monleon D, Montelione GT, Hitchcock-DeGregori SE (2001) Solution NMR structure and folding dynamics of the N terminus of a rat non-muscle alpha-tropomyosin in an engineered chimeric protein. Journal of Molecular Biology, 312(4), 833-47.