NESG statistics





NESG (complete history)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   25,065 39,716 18,504
Expression tested   23,720 37,015 17,338
Expressed (E > 0)   18,253 26,450 13,850
Expressed-Soluble (S > 0)   13,553 17,967 11,114
Expressed-Soluble (ES >= 9)   10,795 14,071 9,011
Fermented   8,692 11,150 7,558
Purification attempted   7,935 9,908 6,975
Purified (>= 0.5 mg yield)   5,832 7,048 5,092
HSQC spectra recorded   2,755 3,524 2,446
"Good" HSQC spectra   1,461 1,833 1,312
HTP crystal screening   2,817 3,339 2,545
Crystal hit   1,467 1,655 1,361
X-Ray structures   597 604 586 652
NMR structures   484 486 476 51343
Total structures   1,038 1,055 1,011 1,165

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[activity table created 2014-04-18]



NESG (PSI-1)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   5,736 7,902 5,556
Expression tested   4,697 5,928 4,568
Expressed (E > 0)   3,321 3,606 3,261
Expressed-Soluble (S > 0)   2,526 2,724 2,498
Expressed-Soluble (ES >= 9)   2,026 2,150 2,009
Fermented   2,412 3,044 2,372
Purification attempted   2,152 2,590 2,119
Purified (>= 0.5 mg yield)   1,083 1,116 1,081
HSQC spectra recorded   791 1,094 788
"Good" HSQC spectra   344 484 342
HTP crystal screening   268 268 268
Crystal hit   146 146 146
X-Ray structures   109 109 109 112
NMR structures   80 80 80 832
Total structures   187 187 187 195

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[activity table created 2014-04-18]



NESG (PSI-2)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   15,302 21,619 11,016
Expression tested   14,490 20,236 10,334
Expressed (E > 0)   10,901 14,393 8,211
Expressed-Soluble (S > 0)   8,242 10,348 6,711
Expressed-Soluble (ES >= 9)   6,540 8,101 5,383
Fermented   4,214 4,999 3,602
Purification attempted   4,232 4,968 3,663
Purified (>= 0.5 mg yield)   3,213 3,771 2,754
HSQC spectra recorded   1,439 1,694 1,239
"Good" HSQC spectra   808 952 712
HTP crystal screening   1,931 2,226 1,727
Crystal hit   1,035 1,149 945
X-Ray structures   372 375 366 397
NMR structures   298 299 294 30931
Total structures   639 649 622 706

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[activity table created 2014-04-18]



NESG (PSI-Biology)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   5,993 12,345 3,985
Expression tested   5,609 11,793 3,689
Expressed (E > 0)   4,874 9,273 3,334
Expressed-Soluble (S > 0)   3,577 5,697 2,749
Expressed-Soluble (ES >= 9)   2,894 4,504 2,317
Fermented   2,594 3,555 2,166
Purification attempted   2,240 2,944 1,930
Purified (>= 0.5 mg yield)   1,948 2,527 1,699
HSQC spectra recorded   632 824 551
"Good" HSQC spectra   364 445 323
HTP crystal screening   707 896 650
Crystal hit   334 393 326
X-Ray structures   125 129 122 143
NMR structures   116 116 113 1216
Total structures   235 240 229 264

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[activity table created 2014-04-19]



Human proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,170 12,743 3,870
Expression tested   5,975 12,371 3,742
Expressed (E > 0)   4,823 9,073 3,126
Expressed-Soluble (S > 0)   3,220 5,271 2,339
Expressed-Soluble (ES >= 9)   2,474 3,957 1,871
Fermented   2,095 2,939 1,629
Purification attempted   1,769 2,395 1,418
Purified (>= 0.5 mg yield)   1,478 1,990 1,204
HSQC spectra recorded   509 709 405
"Good" HSQC spectra   283 365 232
HTP crystal screening   408 582 328
Crystal hit   162 192 140
X-Ray structures   41 44 40 47
NMR structures   90 90 85 918
Total structures   123 128 116 138

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[attrition table created 2014-04-19]



Eukaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   7,415 15,858 4,917
Expression tested   6,947 14,676 4,523
Expressed (E > 0)   5,496 10,547 3,643
Expressed-Soluble (S > 0)   3,708 6,125 2,731
Expressed-Soluble (ES >= 9)   2,841 4,597 2,169
Fermented   2,658 3,919 2,145
Purification attempted   2,322 3,227 1,946
Purified (>= 0.5 mg yield)   1,601 2,190 1,307
HSQC spectra recorded   700 1,070 592
"Good" HSQC spectra   348 484 294
HTP crystal screening   430 609 345
Crystal hit   170 200 148
X-Ray structures   47 50 46 56
NMR structures   116 116 111 1188
Total structures   155 160 148 174

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[attrition table created 2014-04-19]



Prokaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   17,650 23,858 13,588
Expression tested   16,774 22,341 12,816
Expressed (E > 0)   12,758 15,904 10,208
Expressed-Soluble (S > 0)   9,846 11,843 8,384
Expressed-Soluble (ES >= 9)   7,954 9,474 6,843
Fermented   6,034 7,232 5,414
Purification attempted   5,613 6,683 5,030
Purified (>= 0.5 mg yield)   4,232 4,860 3,786
HSQC spectra recorded   2,055 2,454 1,855
"Good" HSQC spectra   1,113 1,349 1,018
HTP crystal screening   2,387 2,731 2,200
Crystal hit   1,297 1,456 1,213
X-Ray structures   550 554 540 597
NMR structures   368 370 365 39535
Total structures   883 895 863 992

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[attrition table created 2014-04-19]



CRSH

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   176 391 142
Expression tested   176 391 142
Expressed (E > 0)   152 304 123
Expressed-Soluble (S > 0)   127 214 110
Expressed-Soluble (ES >= 9)   112 181 101
Fermented   116 163 105
Purification attempted   92 127 85
Purified (>= 0.5 mg yield)   82 112 75
HSQC spectra recorded   18 28 18
"Good" HSQC spectra   10 14 10
HTP crystal screening   60 76 57
Crystal hit   30 37 30
X-Ray structures   6 6 6 6
NMR structures   1 1 1 1
Total structures   7 7 7 7

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[attrition table created 2014-04-19]



PSI-2 BIG domains assigned to NESG




PSI-2 BIG domains



Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   200 458 150
Expression tested   191 424 149
Expressed (E > 0)   152 297 127
Expressed-Soluble (S > 0)   125 216 102
Expressed-Soluble (ES >= 9)   110 181 88
Fermented   80 127 68
Purification attempted   80 118 68
Purified (>= 0.5 mg yield)   60 85 50
HSQC spectra recorded   54 76 46
"Good" HSQC spectra   37 49 31
HTP crystal screening   26 34 23
Crystal hit   12 14 12
X-Ray structures   4 4 4 5
NMR structures   19 19 18 192
Total structures   21 22 20 24

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[attrition table created 2014-04-19]



Human Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   84 216 60
Expression tested   75 195 59
Expressed (E > 0)   63 144 53
Expressed-Soluble (S > 0)   45 85 37
Expressed-Soluble (ES >= 9)   37 67 30
Fermented   32 52 25
Purification attempted   37 56 29
Purified (>= 0.5 mg yield)   26 40 20
HSQC spectra recorded   22 32 18
"Good" HSQC spectra   16 22 13
HTP crystal screening   9 14 7
Crystal hit   4 6 4
X-Ray structures   2 2 2 3
NMR structures   7 7 6 71
Total structures   8 9 7 10

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[attrition table created 2014-04-19]



D. Baker outreach targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   406 420 384
Expression tested   371 380 360
Expressed (E > 0)   333 337 323
Expressed-Soluble (S > 0)   325 328 315
Expressed-Soluble (ES >= 9)   299 301 290
Fermented   350 352 340
Purification attempted   304 304 295
Purified (>= 0.5 mg yield)   291 291 282
HSQC spectra recorded   122 122 116
"Good" HSQC spectra   72 72 67
HTP crystal screening   239 239 231
Crystal hit   132 132 130
X-Ray structures   45 45 44 51
NMR structures   12 12 12 14
Total structures   57 57 56 65

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[attrition table created 2014-04-19]



HCPIN (Human Cancer Interaction Networks)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   3,137 7,865 1,523
Expression tested   3,103 7,769 1,509
Expressed (E > 0)   2,590 5,757 1,393
Expressed-Soluble (S > 0)   1,693 3,192 1,060
Expressed-Soluble (ES >= 9)   1,330 2,418 870
Fermented   992 1,595 679
Purification attempted   822 1,281 577
Purified (>= 0.5 mg yield)   708 1,090 511
HSQC spectra recorded   385 559 298
"Good" HSQC spectra   230 300 186
HTP crystal screening   315 453 249
Crystal hit   131 152 110
X-Ray structures   29 29 28 30
NMR structures   70 70 67 716
Total structures   93 95 88 101

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[attrition table created 2014-04-19]



Mitochondrial Proteome Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   644 1,765 310
Expression tested   622 1,695 297
Expressed (E > 0)   500 1,166 260
Expressed-Soluble (S > 0)   346 642 214
Expressed-Soluble (ES >= 9)   285 511 188
Fermented   245 416 167
Purification attempted   181 300 128
Purified (>= 0.5 mg yield)   167 262 120
HSQC spectra recorded   50 80 40
"Good" HSQC spectra   18 30 13
HTP crystal screening   68 101 55
Crystal hit   18 28 17
X-Ray structures   2 5 2 5
NMR structures   4 4 3 4
Total structures   6 9 5 9

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[attrition table created 2014-04-19]



Mitochondrial Proteome Partnership - nominated targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   307 1,108 131
Expression tested   306 1,103 130
Expressed (E > 0)   259 764 122
Expressed-Soluble (S > 0)   200 429 117
Expressed-Soluble (ES >= 9)   170 336 108
Fermented   157 290 101
Purification attempted   111 200 76
Purified (>= 0.5 mg yield)   98 163 69
HSQC spectra recorded   27 48 22
"Good" HSQC spectra   10 22 7
HTP crystal screening   35 54 27
Crystal hit   9 13 8
X-Ray structures   1 1 1 1
NMR structures   4 4 3 4
Total structures   5 5 4 5

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[attrition table created 2014-04-19]



Chaperone-Enabled Studies of Epigenetic Regulation Enzymes Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   204 867 85
Expression tested   202 855 85
Expressed (E > 0)   182 735 77
Expressed-Soluble (S > 0)   124 315 68
Expressed-Soluble (ES >= 9)   97 233 58
Fermented   95 194 62
Purification attempted   67 129 48
Purified (>= 0.5 mg yield)   53 92 40
HSQC spectra recorded   19 29 16
"Good" HSQC spectra   15 22 13
HTP crystal screening   9 12 9
Crystal hit   4 4 4
X-Ray structures   1 1 1 2
NMR structures   8 8 8 8
Total structures   9 9 9 10

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[attrition table created 2014-04-19]



Protein Homeostatis Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   201 328 90
Expression tested   180 250 79
Expressed (E > 0)   147 196 71
Expressed-Soluble (S > 0)   114 156 62
Expressed-Soluble (ES >= 9)   85 121 48
Fermented   55 67 37
Purification attempted   47 57 31
Purified (>= 0.5 mg yield)   44 52 29
HSQC spectra recorded   6 8 3
"Good" HSQC spectra   2 3 2
HTP crystal screening   19 21 13
Crystal hit   6 8 6
X-Ray structures   0 0 0 0
NMR structures   1 1 1 1
Total structures   1 1 1 1

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[attrition table created 2014-04-18]



Human transcription factors

Production stage NESG
targets
distinct
constructs
UniProt
ids
HUGO
ids

cloned   2,081 3,287 1,453 1,437
Expressed (E > 0)   1,832 2,638 1,327 1,316
Expressed-Soluble (S > 0)   1,376 1,901 1,084 1,068
Expressed-Soluble (ES >= 9)   1,005 1,391 837 833
Purified (>= 0.5 mg yield)   702 828 617 599

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-04-19]



Bet v1-like super family

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   242 335 196
Expression tested   241 331 195
Expressed (E > 0)   206 276 169
Expressed-Soluble (S > 0)   168 218 149
Expressed-Soluble (ES >= 9)   141 177 129
Fermented   117 157 109
Purification attempted   109 140 103
Purified (>= 0.5 mg yield)   102 131 98
HSQC spectra recorded   79 92 78
"Good" HSQC spectra   63 74 63
HTP crystal screening   56 68 56
Crystal hit   37 40 37
X-Ray structures   18 18 18 18
NMR structures   19 19 19 202
Total structures   35 35 35 38

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-04-19]