NESG statistics





NESG (complete history)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   25,490 40,479 18,718
Expression tested   24,181 37,647 17,573
Expressed (E > 0)   18,715 27,046 14,128
Expressed-Soluble (S > 0)   13,855 18,368 11,330
Expressed-Soluble (ES >= 9)   10,991 14,342 9,166
Fermented   9,147 11,679 7,985
Purification attempted   8,384 10,406 7,397
Purified (>= 0.5 mg yield)   6,207 7,467 5,449
HSQC spectra recorded   2,804 3,587 2,489
"Good" HSQC spectra   1,472 1,844 1,321
HTP crystal screening   2,845 3,368 2,573
Crystal hit   1,479 1,669 1,372
X-Ray structures   606 613 595 662
NMR structures   490 492 482 52043
Total structures   1,053 1,070 1,026 1,182

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[activity table created 2014-11-21]



NESG (PSI-1)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   5,740 7,907 5,558
Expression tested   4,697 5,928 4,568
Expressed (E > 0)   3,321 3,606 3,261
Expressed-Soluble (S > 0)   2,526 2,724 2,498
Expressed-Soluble (ES >= 9)   2,026 2,150 2,009
Fermented   2,412 3,044 2,372
Purification attempted   2,154 2,593 2,120
Purified (>= 0.5 mg yield)   1,083 1,116 1,081
HSQC spectra recorded   791 1,094 788
"Good" HSQC spectra   344 484 342
HTP crystal screening   268 268 268
Crystal hit   146 146 146
X-Ray structures   112 112 112 115
NMR structures   80 80 80 832
Total structures   190 190 190 198

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[activity table created 2014-11-21]



NESG (PSI-2)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   15,302 21,621 11,016
Expression tested   14,490 20,236 10,334
Expressed (E > 0)   10,901 14,393 8,211
Expressed-Soluble (S > 0)   8,242 10,348 6,711
Expressed-Soluble (ES >= 9)   6,540 8,101 5,383
Fermented   4,214 4,999 3,602
Purification attempted   4,232 4,968 3,663
Purified (>= 0.5 mg yield)   3,213 3,771 2,754
HSQC spectra recorded   1,439 1,694 1,239
"Good" HSQC spectra   808 952 712
HTP crystal screening   1,932 2,227 1,728
Crystal hit   1,035 1,149 945
X-Ray structures   372 375 366 397
NMR structures   298 299 294 30931
Total structures   639 649 622 706

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[activity table created 2014-11-22]



NESG (PSI-Biology)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,427 13,114 4,211
Expression tested   6,101 12,426 3,953
Expressed (E > 0)   5,351 9,870 3,630
Expressed-Soluble (S > 0)   3,886 6,099 2,975
Expressed-Soluble (ES >= 9)   3,093 4,776 2,477
Fermented   3,051 4,085 2,595
Purification attempted   2,693 3,444 2,356
Purified (>= 0.5 mg yield)   2,325 2,948 2,058
HSQC spectra recorded   683 889 595
"Good" HSQC spectra   375 456 332
HTP crystal screening   736 926 679
Crystal hit   346 407 337
X-Ray structures   132 136 129 150
NMR structures   123 123 120 1286
Total structures   249 254 243 278

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[activity table created 2014-11-22]



Human proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,519 13,255 4,017
Expression tested   6,336 12,798 3,889
Expressed (E > 0)   5,174 9,476 3,312
Expressed-Soluble (S > 0)   3,405 5,494 2,457
Expressed-Soluble (ES >= 9)   2,556 4,073 1,928
Fermented   2,457 3,341 1,970
Purification attempted   2,142 2,792 1,772
Purified (>= 0.5 mg yield)   1,784 2,317 1,499
HSQC spectra recorded   519 727 411
"Good" HSQC spectra   286 368 233
HTP crystal screening   413 587 333
Crystal hit   164 195 142
X-Ray structures   42 45 41 48
NMR structures   90 90 85 918
Total structures   124 129 117 139

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[attrition table created 2014-11-22]



Eukaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   7,775 16,452 5,074
Expression tested   7,309 15,121 4,671
Expressed (E > 0)   5,848 10,968 3,830
Expressed-Soluble (S > 0)   3,894 6,364 2,850
Expressed-Soluble (ES >= 9)   2,924 4,723 2,227
Fermented   3,021 4,339 2,487
Purification attempted   2,696 3,637 2,301
Purified (>= 0.5 mg yield)   1,908 2,530 1,603
HSQC spectra recorded   710 1,091 598
"Good" HSQC spectra   351 487 295
HTP crystal screening   436 615 351
Crystal hit   172 203 150
X-Ray structures   48 51 47 57
NMR structures   116 116 111 1188
Total structures   156 161 149 175

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[attrition table created 2014-11-22]



Prokaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   17,715 24,027 13,645
Expression tested   16,872 22,527 12,903
Expressed (E > 0)   12,867 16,078 10,299
Expressed-Soluble (S > 0)   9,961 12,004 8,481
Expressed-Soluble (ES >= 9)   8,067 9,619 6,940
Fermented   6,126 7,341 5,499
Purification attempted   5,688 6,771 5,097
Purified (>= 0.5 mg yield)   4,300 4,939 3,847
HSQC spectra recorded   2,094 2,496 1,892
"Good" HSQC spectra   1,121 1,357 1,026
HTP crystal screening   2,409 2,754 2,222
Crystal hit   1,307 1,467 1,222
X-Ray structures   558 562 548 606
NMR structures   374 376 371 40235
Total structures   897 909 877 1,008

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[attrition table created 2014-11-22]



CRSH

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   176 395 142
Expression tested   176 394 142
Expressed (E > 0)   152 307 123
Expressed-Soluble (S > 0)   127 216 110
Expressed-Soluble (ES >= 9)   112 183 101
Fermented   116 166 105
Purification attempted   92 130 85
Purified (>= 0.5 mg yield)   82 113 75
HSQC spectra recorded   18 28 18
"Good" HSQC spectra   10 14 10
HTP crystal screening   60 77 57
Crystal hit   32 40 32
X-Ray structures   7 7 7 7
NMR structures   1 1 1 1
Total structures   8 8 8 8

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[attrition table created 2014-11-22]



PSI-2 BIG domains assigned to NESG




PSI-2 BIG domains



Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   200 460 150
Expression tested   191 425 149
Expressed (E > 0)   152 298 127
Expressed-Soluble (S > 0)   125 217 102
Expressed-Soluble (ES >= 9)   110 182 88
Fermented   80 128 68
Purification attempted   80 119 68
Purified (>= 0.5 mg yield)   60 86 50
HSQC spectra recorded   54 76 46
"Good" HSQC spectra   37 49 31
HTP crystal screening   26 34 23
Crystal hit   12 14 12
X-Ray structures   4 4 4 5
NMR structures   19 19 18 192
Total structures   21 22 20 24

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[attrition table created 2014-11-22]



Human Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   84 218 60
Expression tested   75 196 59
Expressed (E > 0)   63 145 53
Expressed-Soluble (S > 0)   45 86 37
Expressed-Soluble (ES >= 9)   37 68 30
Fermented   32 53 25
Purification attempted   37 57 29
Purified (>= 0.5 mg yield)   26 41 20
HSQC spectra recorded   22 32 18
"Good" HSQC spectra   16 22 13
HTP crystal screening   9 14 7
Crystal hit   4 6 4
X-Ray structures   2 2 2 3
NMR structures   7 7 6 71
Total structures   8 9 7 10

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[attrition table created 2014-11-22]



D. Baker outreach targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   414 428 392
Expression tested   379 388 368
Expressed (E > 0)   340 344 330
Expressed-Soluble (S > 0)   332 335 322
Expressed-Soluble (ES >= 9)   306 308 297
Fermented   359 361 349
Purification attempted   313 313 304
Purified (>= 0.5 mg yield)   299 299 290
HSQC spectra recorded   137 137 131
"Good" HSQC spectra   76 76 71
HTP crystal screening   249 249 241
Crystal hit   135 135 133
X-Ray structures   51 51 50 57
NMR structures   17 17 17 19
Total structures   68 68 67 76

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[attrition table created 2014-11-22]



HCPIN (Human Cancer Interaction Networks)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   3,216 8,000 1,545
Expression tested   3,185 7,900 1,534
Expressed (E > 0)   2,670 5,881 1,426
Expressed-Soluble (S > 0)   1,742 3,275 1,084
Expressed-Soluble (ES >= 9)   1,354 2,469 884
Fermented   1,072 1,704 748
Purification attempted   911 1,390 657
Purified (>= 0.5 mg yield)   781 1,181 578
HSQC spectra recorded   391 571 303
"Good" HSQC spectra   230 300 186
HTP crystal screening   315 453 249
Crystal hit   131 153 110
X-Ray structures   29 29 28 30
NMR structures   70 70 67 716
Total structures   93 95 88 101

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[attrition table created 2014-11-22]



Mitochondrial Proteome Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   647 1,798 311
Expression tested   625 1,705 298
Expressed (E > 0)   503 1,175 261
Expressed-Soluble (S > 0)   351 652 216
Expressed-Soluble (ES >= 9)   290 520 190
Fermented   252 425 170
Purification attempted   191 314 136
Purified (>= 0.5 mg yield)   175 274 127
HSQC spectra recorded   53 86 40
"Good" HSQC spectra   21 33 14
HTP crystal screening   74 107 61
Crystal hit   19 29 18
X-Ray structures   3 6 3 6
NMR structures   4 4 3 4
Total structures   7 10 6 10

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[attrition table created 2014-11-22]



Mitochondrial Proteome Partnership - nominated targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   307 1,108 131
Expression tested   306 1,103 130
Expressed (E > 0)   259 764 122
Expressed-Soluble (S > 0)   201 430 117
Expressed-Soluble (ES >= 9)   171 337 108
Fermented   158 291 101
Purification attempted   114 204 77
Purified (>= 0.5 mg yield)   100 166 70
HSQC spectra recorded   29 53 22
"Good" HSQC spectra   10 22 7
HTP crystal screening   36 55 28
Crystal hit   9 13 8
X-Ray structures   2 2 2 2
NMR structures   4 4 3 4
Total structures   6 6 5 6

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[attrition table created 2014-11-22]



Chaperone-Enabled Studies of Epigenetic Regulation Enzymes Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   210 866 86
Expression tested   208 861 86
Expressed (E > 0)   187 740 78
Expressed-Soluble (S > 0)   127 318 70
Expressed-Soluble (ES >= 9)   99 235 59
Fermented   101 200 66
Purification attempted   75 137 55
Purified (>= 0.5 mg yield)   58 97 45
HSQC spectra recorded   19 30 16
"Good" HSQC spectra   15 22 13
HTP crystal screening   9 12 9
Crystal hit   4 4 4
X-Ray structures   1 1 1 2
NMR structures   8 8 8 8
Total structures   9 9 9 10

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-11-22]



Protein Homeostatis Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   204 359 90
Expression tested   183 262 79
Expressed (E > 0)   150 207 71
Expressed-Soluble (S > 0)   118 166 63
Expressed-Soluble (ES >= 9)   89 130 49
Fermented   61 76 39
Purification attempted   53 67 36
Purified (>= 0.5 mg yield)   49 61 33
HSQC spectra recorded   7 9 3
"Good" HSQC spectra   5 6 3
HTP crystal screening   24 26 18
Crystal hit   7 9 7
X-Ray structures   0 0 0 0
NMR structures   1 1 1 1
Total structures   1 1 1 1

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-11-21]



Human transcription factors

Production stage NESG
targets
distinct
constructs
UniProt
ids
HUGO
ids

cloned   2,611 4,146 1,689 1,665
Expressed (E > 0)   2,310 3,304 1,580 1,558
Expressed-Soluble (S > 0)   1,631 2,240 1,250 1,244
Expressed-Soluble (ES >= 9)   1,140 1,592 935 932
Purified (>= 0.5 mg yield)   1,026 1,172 924 913

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-11-22]



Bet v1-like super family

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   242 335 196
Expression tested   241 331 195
Expressed (E > 0)   206 276 169
Expressed-Soluble (S > 0)   168 218 149
Expressed-Soluble (ES >= 9)   141 177 129
Fermented   117 157 109
Purification attempted   109 140 103
Purified (>= 0.5 mg yield)   102 131 98
HSQC spectra recorded   79 92 78
"Good" HSQC spectra   63 74 63
HTP crystal screening   56 68 56
Crystal hit   37 40 37
X-Ray structures   18 18 18 18
NMR structures   20 20 20 212
Total structures   36 36 36 39

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-11-22]