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NESG statistics (data retrieved from targetdb and updated weekly) PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   12,466 21,190 Expressed (E > 0)   9,371 14,544 Expressed-Soluble (ES >= 10)   5,484 7,615 Expressed-Insoluble   3,887 6,929 Purified (>= 0.5 mg yield)   2,819 3,624 HSQC spectra recorded   1,176 1,551 "Good" or "Promising" HSQC spectra   654 870 Crystal hits   925 1,102 Diffraction data collected   366 425 Strong-diffracting crystals (< 4 A)   323 377 X-ray crystal structures in PDB   414 NMR structures in PDB   316 Resonance assignment to BMRB   316 Distinct targets in PDB   659 Total structures in PDB   730 PSI-1 & PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   17,671 28,490 Expressed (E > 0)   12,341 18,006 Expressed-Soluble (ES >= 10)   7,215 9,583 Expressed-Insoluble   5,126 8,423 Purified (>= 0.5 mg yield)   3,911 4,799 HSQC spectra recorded   1,982 2,664 "Good" or "Promising" HSQC spectra   1,009 1,376 Crystal hits   1,144 1,347 Diffraction data collected   517 601 Strong-diffracting crystals (< 4 A)   467 546 X-ray crystal structures in PDB   523 NMR structures in PDB   399 Resonance assignment to BMRB   399 Distinct targets in PDB   834 Total structures in PDB   922 PSI-2 assigned BIG Families NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   5,470 8,233 Expressed (E > 0)   3,979 5,621 Expressed-Soluble (ES >= 10)   2,159 2,821 Expressed-Insoluble   1,820 2,800 Purified (>= 0.5 mg yield)   1,087 1,309 HSQC spectra recorded   500 603 "Good" or "Promising" HSQC spectra   290 351 Crystal hits   327 377 Diffraction data collected   128 145 Strong-diffracting crystals (< 4 A)   105 121 X-ray crystal structures in PDB   374 NMR structures in PDB   263 Distinct targets in PDB   741 Total structures in PDB   637 Structured BIG Families   563 Structured BIG Families assigned to NESG   110 HCPIN (Human Cancer Protein Interaction Network) PSI-1 and PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   1,190 2,741 Expressed (E > 0)   902 1,839 Expressed-Soluble (ES >= 10)   461 800 Expressed-Insoluble   441 1,039 Purified (>= 0.5 mg yield)   222 349 HSQC spectra recorded   127 192 "Good" or "Promising" HSQC spectra   78 117 Crystal hits   70 85 Diffraction data collected   11 13 Strong-diffracting crystals (< 4 A)   11 13 X-ray crystal structures in PDB   21 NMR structures in PDB   31 Resonance assignment to BMRB   31 Distinct targets in PDB   45 Total structures in PDB   52 Human PSI-1 and PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   2,342 4,335 Expressed (E > 0)   1,513 2,642 Expressed-Soluble (ES >= 10)   733 1,130 Expressed-Insoluble   780 1,512 Purified (>= 0.5 mg yield)   416 574 HSQC spectra recorded   189 271 "Good" or "Promising" HSQC spectra   101 151 Crystal hits   84 104 Diffraction data collected   17 24 Strong-diffracting crystals (< 4 A)   17 24 X-ray crystal structures in PDB   29 NMR structures in PDB   42 Resonance assignment to BMRB   42 Distinct targets in PDB   62 Total structures in PDB   71 Human PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   1,382 3,204 Expressed (E > 0)   1,019 2,095 Expressed-Soluble (ES >= 10)   520 901 Expressed-Insoluble   499 1,194 Purified (>= 0.5 mg yield)   251 397 HSQC spectra recorded   134 211 "Good" or "Promising" HSQC spectra   81 129 Crystal hits   75 91 Diffraction data collected   12 15 Strong-diffracting crystals (< 4 A)   12 15 X-ray crystal structures in PDB   23 NMR structures in PDB   39 Resonance assignment to BMRB   39 Distinct targets in PDB   54 Total structures in PDB   62 Eukaryotic PSI-1 and PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   3,164 6,063 Expressed (E > 0)   1,814 3,165 Expressed-Soluble (ES >= 10)   874 1,399 Expressed-Insoluble   940 1,766 Purified (>= 0.5 mg yield)   468 647 HSQC spectra recorded   344 541 "Good" or "Promising" HSQC spectra   144 226 Crystal hits   89 111 Diffraction data collected   20 29 Strong-diffracting crystals (< 4 A)   20 29 X-ray crystal structures in PDB   37 NMR structures in PDB   60 Resonance assignment to BMRB   60 Distinct targets in PDB   85 Total structures in PDB   97 Eukaryotic PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   1,478 3,499 Expressed (E > 0)   1,078 2,210 Expressed-Soluble (ES >= 10)   556 964 Expressed-Insoluble   522 1,246 Purified (>= 0.5 mg yield)   270 418 HSQC spectra recorded   143 224 "Good" or "Promising" HSQC spectra   87 137 Crystal hits   78 94 Diffraction data collected   13 16 Strong-diffracting crystals (< 4 A)   13 16 X-ray crystal structures in PDB   25 NMR structures in PDB   51 Resonance assignment to BMRB   51 Distinct targets in PDB   67 Total structures in PDB   76 Prokaryotic PSI-1 and PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   14,593 22,770 Expressed (E > 0)   10,585 14,952 Expressed-Soluble (ES >= 10)   6,378 8,257 Expressed-Insoluble   4,207 6,695 Purified (>= 0.5 mg yield)   3,453 4,165 HSQC spectra recorded   1,704 2,244 "Good" or "Promising" HSQC spectra   892 1,200 Crystal hits   1,057 1,238 Diffraction data collected   499 574 Strong-diffracting crystals (< 4 A)   449 519 X-ray crystal structures in PDB   486 NMR structures in PDB   339 Resonance assignment to BMRB   339 Distinct targets in PDB   749 Total structures in PDB   825 Prokaryotic PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   10,989 17,677 Expressed (E > 0)   8,294 12,325 Expressed-Soluble (ES >= 10)   4,929 6,646 Expressed-Insoluble   3,365 5,679 Purified (>= 0.5 mg yield)   2,550 3,206 HSQC spectra recorded   1,034 1,327 "Good" or "Promising" HSQC spectra   568 733 Crystal hits   847 1,008 Diffraction data collected   353 409 Strong-diffracting crystals (< 4 A)   310 361 X-ray crystal structures in PDB   389 NMR structures in PDB   265 Resonance assignment to BMRB   265 Distinct targets in PDB   592 Total structures in PDB   654 PSI-1 and PSI-2 Salmonella Protein Production Statistics (NESG Orgs: St, Sl, Sc) Production stage number of targets number of constructs Cloned   356 449 Expressed (E > 0)   299 361 Expressed-Soluble (ES >= 10)   181 212 Expressed-Insoluble   118 149 Purified (>= 0.5 mg yield)   111 124 HSQC spectra recorded   44 48 "Good" or "Promising" HSQC spectra   28 28 Crystal hits   30 33 Diffraction data collected   14 15 Strong-diffracting crystals (< 4 A)   13 14 X-ray crystal structures in PDB   10 NMR structures in PDB   11 Resonance assignment to BMRB   11 Distinct targets in PDB   17 Total structures in PDB   21 PSI-1 and PSI-2 transcription factors Production stage number of targets number of constructs Cloned   207 443 Expressed (E > 0)   145 278 Expressed-Soluble (ES >= 10)   91 160 Expressed-Insoluble   54 118 Purified (>= 0.5 mg yield)   37 55 HSQC spectra recorded   31 40 "Good" or "Promising" HSQC spectra   18 25 Crystal hits   13 13 Diffraction data collected   0 0 Strong-diffracting crystals (< 4 A)   0 0 X-ray crystal structures in PDB   3 NMR structures in PDB   3 Resonance assignment to BMRB   3 Distinct targets in PDB   5 Total structures in PDB   6 OB fold (2.40.50.140) PSI-1 and PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   331 502 Expressed (E > 0)   273 370 Expressed-Soluble (ES >= 10)   213 277 Expressed-Insoluble   60 93 Purified (>= 0.5 mg yield)   128 156 HSQC spectra recorded   70 89 "Good" or "Promising" HSQC spectra   35 45 Crystal hits   35 39 Diffraction data collected   15 16 Strong-diffracting crystals (< 4 A)   10 11 X-ray crystal structures in PDB   12 NMR structures in PDB   14 Resonance assignment to BMRB   14 Distinct targets in PDB   23 Total structures in PDB   26 OB fold (2.40.50.140) PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   315 477 Expressed (E > 0)   263 357 Expressed-Soluble (ES >= 10)   206 268 Expressed-Insoluble   57 89 Purified (>= 0.5 mg yield)   123 151 HSQC spectra recorded   62 78 "Good" or "Promising" HSQC spectra   28 35 Crystal hits   35 39 Diffraction data collected   15 16 Strong-diffracting crystals (< 4 A)   10 11 X-ray crystal structures in PDB   12 NMR structures in PDB   11 Resonance assignment to BMRB   11 Distinct targets in PDB   20 Total structures in PDB   23 Oxidoreductase (3.50.50.60) PSI-1 and PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   633 727 Expressed (E > 0)   468 533 Expressed-Soluble (ES >= 10)   221 245 Expressed-Insoluble   247 288 Purified (>= 0.5 mg yield)   115 126 HSQC spectra recorded   3 4 "Good" or "Promising" HSQC spectra   0 0 Crystal hits   34 36 Diffraction data collected   12 12 Strong-diffracting crystals (< 4 A)   12 12 X-ray crystal structures in PDB   11 NMR structures in PDB   0 Resonance assignment to BMRB   0 Distinct targets in PDB   11 Total structures in PDB   11 Oxidoreductase (3.50.50.60) PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   605 694 Expressed (E > 0)   453 517 Expressed-Soluble (ES >= 10)   216 239 Expressed-Insoluble   237 278 Purified (>= 0.5 mg yield)   110 120 HSQC spectra recorded   2 2 "Good" or "Promising" HSQC spectra   0 0 Crystal hits   33 35 Diffraction data collected   11 11 Strong-diffracting crystals (< 4 A)   11 11 X-ray crystal structures in PDB   11 NMR structures in PDB   0 Resonance assignment to BMRB   0 Distinct targets in PDB   11 Total structures in PDB   11 Ubiquitin (3.10.20.90) PSI-1 and PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   61 163 Expressed (E > 0)   50 104 Expressed-Soluble (ES >= 10)   33 58 Expressed-Insoluble   17 46 Purified (>= 0.5 mg yield)   18 27 HSQC spectra recorded   17 22 "Good" or "Promising" HSQC spectra   14 19 Crystal hits   5 7 Diffraction data collected   0 0 Strong-diffracting crystals (< 4 A)   0 0 X-ray crystal structures in PDB   1 NMR structures in PDB   13 Resonance assignment to BMRB   13 Distinct targets in PDB   13 Total structures in PDB   14 Ubiquitin (3.10.20.90) PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   52 140 Expressed (E > 0)   43 88 Expressed-Soluble (ES >= 10)   27 46 Expressed-Insoluble   16 42 Purified (>= 0.5 mg yield)   16 24 HSQC spectra recorded   12 16 "Good" or "Promising" HSQC spectra   10 14 Crystal hits   5 7 Diffraction data collected   0 0 Strong-diffracting crystals (< 4 A)   0 0 X-ray crystal structures in PDB   1 NMR structures in PDB   11 Resonance assignment to BMRB   11 Distinct targets in PDB   11 Total structures in PDB   12 Start domain (3.30.530.20) PSI-1 and PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   196 288 Expressed (E > 0)   167 230 Expressed-Soluble (ES >= 10)   124 150 Expressed-Insoluble   43 80 Purified (>= 0.5 mg yield)   66 73 HSQC spectra recorded   24 24 "Good" or "Promising" HSQC spectra   17 17 Crystal hits   13 13 Diffraction data collected   6 6 Strong-diffracting crystals (< 4 A)   6 6 X-ray crystal structures in PDB   7 NMR structures in PDB   8 Resonance assignment to BMRB   8 Distinct targets in PDB   13 Total structures in PDB   15 Start domain (3.30.530.20) PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   166 253 Expressed (E > 0)   145 208 Expressed-Soluble (ES >= 10)   105 131 Expressed-Insoluble   40 77 Purified (>= 0.5 mg yield)   55 62 HSQC spectra recorded   15 15 "Good" or "Promising" HSQC spectra   8 8 Crystal hits   9 9 Diffraction data collected   4 4 Strong-diffracting crystals (< 4 A)   4 4 X-ray crystal structures in PDB   4 NMR structures in PDB   5 Resonance assignment to BMRB   5 Distinct targets in PDB   7 Total structures in PDB   9 Tol-B (2.120.10.30) PSI-1 and PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   144 261 Expressed (E > 0)   116 192 Expressed-Soluble (ES >= 10)   58 83 Expressed-Insoluble   58 109 Purified (>= 0.5 mg yield)   31 38 HSQC spectra recorded   0 0 "Good" or "Promising" HSQC spectra   0 0 Crystal hits   15 15 Diffraction data collected   6 6 Strong-diffracting crystals (< 4 A)   6 6 X-ray crystal structures in PDB   6 NMR structures in PDB   0 Resonance assignment to BMRB   0 Distinct targets in PDB   6 Total structures in PDB   6 Tol-B (2.120.10.30) PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   141 257 Expressed (E > 0)   115 191 Expressed-Soluble (ES >= 10)   57 82 Expressed-Insoluble   58 109 Purified (>= 0.5 mg yield)   31 38 HSQC spectra recorded   0 0 "Good" or "Promising" HSQC spectra   0 0 Crystal hits   15 15 Diffraction data collected   6 6 Strong-diffracting crystals (< 4 A)   6 6 X-ray crystal structures in PDB   6 NMR structures in PDB   0 Resonance assignment to BMRB   0 Distinct targets in PDB   6 Total structures in PDB   6 Ubiquitin E2 (3.10.110.10) PSI-1 and PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   12 22 Expressed (E > 0)   7 10 Expressed-Soluble (ES >= 10)   2 3 Expressed-Insoluble   5 7 Purified (>= 0.5 mg yield)   2 3 HSQC spectra recorded   0 0 "Good" or "Promising" HSQC spectra   0 0 Crystal hits   0 0 Diffraction data collected   0 0 Strong-diffracting crystals (< 4 A)   0 0 X-ray crystal structures in PDB   0 NMR structures in PDB   0 Resonance assignment to BMRB   0 Distinct targets in PDB   0 Total structures in PDB   0 Ubiquitin E2 (3.10.110.10) PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   5 10 Expressed (E > 0)   4 6 Expressed-Soluble (ES >= 10)   2 3 Expressed-Insoluble   2 3 Purified (>= 0.5 mg yield)   0 0 HSQC spectra recorded   0 0 "Good" or "Promising" HSQC spectra   0 0 Crystal hits   0 0 Diffraction data collected   0 0 Strong-diffracting crystals (< 4 A)   0 0 X-ray crystal structures in PDB   0 NMR structures in PDB   0 Resonance assignment to BMRB   0 Distinct targets in PDB   0 Total structures in PDB   0 Polysaccharide Biosynthesis (3.90.550.10) PSI-1 and PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   449 524 Expressed (E > 0)   315 355 Expressed-Soluble (ES >= 10)   188 192 Expressed-Insoluble   127 163 Purified (>= 0.5 mg yield)   40 40 HSQC spectra recorded   0 0 "Good" or "Promising" HSQC spectra   0 0 Crystal hits   9 9 Diffraction data collected   3 3 Strong-diffracting crystals (< 4 A)   2 2 X-ray crystal structures in PDB   5 NMR structures in PDB   0 Resonance assignment to BMRB   0 Distinct targets in PDB   4 Total structures in PDB   5 Polysaccharide Biosynthesis (3.90.550.10) PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   443 516 Expressed (E > 0)   310 349 Expressed-Soluble (ES >= 10)   186 190 Expressed-Insoluble   124 159 Purified (>= 0.5 mg yield)   38 38 HSQC spectra recorded   0 0 "Good" or "Promising" HSQC spectra   0 0 Crystal hits   8 8 Diffraction data collected   2 2 Strong-diffracting crystals (< 4 A)   1 1 X-ray crystal structures in PDB   4 NMR structures in PDB   0 Resonance assignment to BMRB   0 Distinct targets in PDB   3 Total structures in PDB   4 P-loop (3.40.50.300) PSI-1 and PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   860 1,186 Expressed (E > 0)   648 847 Expressed-Soluble (ES >= 10)   377 409 Expressed-Insoluble   271 438 Purified (>= 0.5 mg yield)   212 225 HSQC spectra recorded   37 40 "Good" or "Promising" HSQC spectra   11 12 Crystal hits   62 65 Diffraction data collected   26 27 Strong-diffracting crystals (< 4 A)   20 21 X-ray crystal structures in PDB   16 NMR structures in PDB   2 Resonance assignment to BMRB   2 Distinct targets in PDB   15 Total structures in PDB   18 P-loop (3.40.50.300) PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   704 988 Expressed (E > 0)   551 745 Expressed-Soluble (ES >= 10)   315 347 Expressed-Insoluble   236 398 Purified (>= 0.5 mg yield)   164 177 HSQC spectra recorded   11 12 "Good" or "Promising" HSQC spectra   6 7 Crystal hits   50 53 Diffraction data collected   18 19 Strong-diffracting crystals (< 4 A)   13 14 X-ray crystal structures in PDB   13 NMR structures in PDB   2 Resonance assignment to BMRB   2 Distinct targets in PDB   12 Total structures in PDB   15 NAD(P) binding domain (3.40.50.720) PSI-1 and PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   824 1,000 Expressed (E > 0)   621 735 Expressed-Soluble (ES >= 10)   335 383 Expressed-Insoluble   286 352 Purified (>= 0.5 mg yield)   186 211 HSQC spectra recorded   17 18 "Good" or "Promising" HSQC spectra   7 7 Crystal hits   60 68 Diffraction data collected   29 34 Strong-diffracting crystals (< 4 A)   28 32 X-ray crystal structures in PDB   26 NMR structures in PDB   1 Resonance assignment to BMRB   1 Distinct targets in PDB   25 Total structures in PDB   27 NAD(P) binding domain (3.40.50.720) PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   733 884 Expressed (E > 0)   565 673 Expressed-Soluble (ES >= 10)   298 343 Expressed-Insoluble   267 330 Purified (>= 0.5 mg yield)   162 183 HSQC spectra recorded   8 8 "Good" or "Promising" HSQC spectra   6 6 Crystal hits   54 61 Diffraction data collected   25 29 Strong-diffracting crystals (< 4 A)   24 27 X-ray crystal structures in PDB   24 NMR structures in PDB   1 Resonance assignment to BMRB   1 Distinct targets in PDB   23 Total structures in PDB   25 Aldolase class I (3.20.20.70) PSI-1 and PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   324 467 Expressed (E > 0)   228 301 Expressed-Soluble (ES >= 10)   125 138 Expressed-Insoluble   103 163 Purified (>= 0.5 mg yield)   73 79 HSQC spectra recorded   11 12 "Good" or "Promising" HSQC spectra   3 3 Crystal hits   20 21 Diffraction data collected   11 12 Strong-diffracting crystals (< 4 A)   10 11 X-ray crystal structures in PDB   11 NMR structures in PDB   0 Resonance assignment to BMRB   0 Distinct targets in PDB   11 Total structures in PDB   11 Aldolase class I (3.20.20.70) PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   177 261 Expressed (E > 0)   145 207 Expressed-Soluble (ES >= 10)   78 88 Expressed-Insoluble   67 119 Purified (>= 0.5 mg yield)   47 52 HSQC spectra recorded   3 4 "Good" or "Promising" HSQC spectra   1 1 Crystal hits   12 12 Diffraction data collected   5 5 Strong-diffracting crystals (< 4 A)   5 5 X-ray crystal structures in PDB   5 NMR structures in PDB   0 Resonance assignment to BMRB   0 Distinct targets in PDB   5 Total structures in PDB   5 Vaccinia Virus protein VP39 (3.40.50.150) PSI-1 and PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   360 565 Expressed (E > 0)   277 392 Expressed-Soluble (ES >= 10)   174 204 Expressed-Insoluble   103 188 Purified (>= 0.5 mg yield)   93 103 HSQC spectra recorded   19 19 "Good" or "Promising" HSQC spectra   9 9 Crystal hits   32 36 Diffraction data collected   18 19 Strong-diffracting crystals (< 4 A)   15 15 X-ray crystal structures in PDB   19 NMR structures in PDB   1 Resonance assignment to BMRB   1 Distinct targets in PDB   18 Total structures in PDB   20 Vaccinia Virus protein VP39 (3.40.50.150) PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   254 423 Expressed (E > 0)   208 317 Expressed-Soluble (ES >= 10)   143 172 Expressed-Insoluble   65 145 Purified (>= 0.5 mg yield)   72 81 HSQC spectra recorded   9 9 "Good" or "Promising" HSQC spectra   5 5 Crystal hits   28 32 Diffraction data collected   16 17 Strong-diffracting crystals (< 4 A)   13 13 X-ray crystal structures in PDB   15 NMR structures in PDB   1 Resonance assignment to BMRB   1 Distinct targets in PDB   14 Total structures in PDB   16 Metagenomic targets PSI-2 NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   1,056 1,801 Expressed (E > 0)   887 1,390 Expressed-Soluble (ES >= 10)   594 838 Expressed-Insoluble   293 552 Purified (>= 0.5 mg yield)   342 426 HSQC spectra recorded   111 131 "Good" or "Promising" HSQC spectra   75 87 Crystal hits   110 121 Diffraction data collected   36 37 Strong-diffracting crystals (< 4 A)   35 36 X-ray crystal structures in PDB   39 NMR structures in PDB   25 Resonance assignment to BMRB   25 Distinct targets in PDB   56 Total structures in PDB   64 MCSG salvage targets NESG Protein Production Statistics Production stage number of targets number of constructs Cloned   53 60 Expressed (E > 0)   47 49 Expressed-Soluble (ES >= 10)   34 36 Expressed-Insoluble   13 13 Purified (>= 0.5 mg yield)   28 30 HSQC spectra recorded   26 26 "Good" or "Promising" HSQC spectra   13 13 Crystal hits   7 7 Diffraction data collected   3 3 Strong-diffracting crystals (< 4 A)   3 3 X-ray crystal structures in PDB   3 NMR structures in PDB   7 Resonance assignment to BMRB   7 Distinct targets in PDB   7 Total structures in PDB   10 Year 5 BIG OPEN project (2009-03-01 - present) Production stage number of targets number of constructs Cloned   1,569 3,647 Expressed (E > 0)   1,136 2,333 Expressed-Soluble (ES >= 10)   598 1,042 Expressed-Insoluble   538 1,291 Purified (>= 0.5 mg yield)   272 417 HSQC spectra recorded   120 165 "Good" or "Promising" HSQC spectra   78 99 Crystal hits   93 119 Diffraction data collected   17 23 Strong-diffracting crystals (< 4 A)   16 22 X-ray crystal structures in PDB   29 NMR structures in PDB   30 Resonance assignment to BMRB   30 Distinct targets in PDB   53 Total structures in PDB   59