NESG statistics





NESG (complete history)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   25,775 40,859 18,902
Expression tested   24,431 38,035 17,725
Expressed (E > 0)   18,948 27,407 14,274
Expressed-Soluble (S > 0)   13,932 18,536 11,389
Expressed-Soluble (ES >= 9)   11,046 14,465 9,211
Fermented   9,392 11,987 8,211
Purification attempted   8,643 10,718 7,639
Purified (>= 0.5 mg yield)   6,447 7,758 5,680
HSQC spectra recorded   2,815 3,598 2,500
"Good" HSQC spectra   1,475 1,847 1,322
HTP crystal screening   2,845 3,370 2,573
Crystal hit   1,480 1,672 1,373
X-Ray structures   608 615 597 664
NMR structures   496 498 488 53543
Total structures   1,061 1,078 1,034 1,199

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[activity table created 2015-08-21]



NESG (PSI-1)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   5,754 7,921 5,569
Expression tested   4,698 5,929 4,569
Expressed (E > 0)   3,321 3,606 3,261
Expressed-Soluble (S > 0)   2,526 2,724 2,498
Expressed-Soluble (ES >= 9)   2,026 2,150 2,009
Fermented   2,412 3,044 2,372
Purification attempted   2,167 2,606 2,131
Purified (>= 0.5 mg yield)   1,095 1,128 1,093
HSQC spectra recorded   791 1,094 788
"Good" HSQC spectra   344 484 342
HTP crystal screening   268 268 268
Crystal hit   146 146 146
X-Ray structures   112 112 112 115
NMR structures   80 80 80 832
Total structures   190 190 190 198

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[activity table created 2015-08-21]



NESG (PSI-2)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   15,302 21,621 11,016
Expression tested   14,490 20,236 10,334
Expressed (E > 0)   10,901 14,393 8,211
Expressed-Soluble (S > 0)   8,242 10,348 6,711
Expressed-Soluble (ES >= 9)   6,540 8,101 5,383
Fermented   4,214 4,999 3,602
Purification attempted   4,232 4,968 3,663
Purified (>= 0.5 mg yield)   3,213 3,771 2,754
HSQC spectra recorded   1,439 1,694 1,239
"Good" HSQC spectra   808 952 712
HTP crystal screening   1,932 2,227 1,728
Crystal hit   1,035 1,149 945
X-Ray structures   372 375 366 397
NMR structures   298 299 294 30931
Total structures   639 649 622 706

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[activity table created 2015-08-21]



NESG (PSI-Biology)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,717 13,501 4,415
Expression tested   6,354 12,817 4,119
Expressed (E > 0)   5,587 10,234 3,788
Expressed-Soluble (S > 0)   3,965 6,270 3,037
Expressed-Soluble (ES >= 9)   3,150 4,901 2,526
Fermented   3,298 4,395 2,827
Purification attempted   2,948 3,752 2,599
Purified (>= 0.5 mg yield)   2,562 3,236 2,287
HSQC spectra recorded   694 900 606
"Good" HSQC spectra   378 459 333
HTP crystal screening   736 928 679
Crystal hit   347 410 338
X-Ray structures   134 138 131 152
NMR structures   134 134 131 1436
Total structures   262 267 256 295

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[activity table created 2015-08-22]



Human proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,777 13,587 4,174
Expression tested   6,541 13,070 3,996
Expressed (E > 0)   5,378 9,744 3,429
Expressed-Soluble (S > 0)   3,459 5,609 2,493
Expressed-Soluble (ES >= 9)   2,591 4,159 1,953
Fermented   2,662 3,587 2,156
Purification attempted   2,364 3,050 1,977
Purified (>= 0.5 mg yield)   1,988 2,555 1,694
HSQC spectra recorded   522 730 414
"Good" HSQC spectra   286 368 233
HTP crystal screening   413 587 333
Crystal hit   164 195 142
X-Ray structures   42 45 41 48
NMR structures   91 91 86 928
Total structures   125 130 118 140

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[attrition table created 2015-08-22]



Eukaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   8,033 16,787 5,231
Expression tested   7,524 15,450 4,788
Expressed (E > 0)   6,062 11,289 3,957
Expressed-Soluble (S > 0)   3,954 6,503 2,892
Expressed-Soluble (ES >= 9)   2,963 4,821 2,256
Fermented   3,233 4,599 2,680
Purification attempted   2,918 3,906 2,506
Purified (>= 0.5 mg yield)   2,112 2,779 1,798
HSQC spectra recorded   713 1,094 601
"Good" HSQC spectra   351 487 295
HTP crystal screening   436 615 351
Crystal hit   172 203 150
X-Ray structures   48 51 47 57
NMR structures   117 117 112 1208
Total structures   157 162 150 177

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[attrition table created 2015-08-22]



Prokaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   17,742 24,072 13,672
Expression tested   16,907 22,587 12,938
Expressed (E > 0)   12,886 16,119 10,318
Expressed-Soluble (S > 0)   9,978 12,034 8,498
Expressed-Soluble (ES >= 9)   8,083 9,645 6,956
Fermented   6,159 7,390 5,532
Purification attempted   5,725 6,814 5,134
Purified (>= 0.5 mg yield)   4,336 4,981 3,883
HSQC spectra recorded   2,102 2,504 1,900
"Good" HSQC spectra   1,124 1,360 1,027
HTP crystal screening   2,409 2,756 2,222
Crystal hit   1,308 1,470 1,223
X-Ray structures   560 564 550 608
NMR structures   379 381 376 41535
Total structures   904 916 884 1,023

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[attrition table created 2015-08-22]



CRSH

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   176 395 142
Expression tested   176 395 142
Expressed (E > 0)   152 308 123
Expressed-Soluble (S > 0)   127 217 110
Expressed-Soluble (ES >= 9)   112 184 101
Fermented   116 167 105
Purification attempted   92 131 85
Purified (>= 0.5 mg yield)   82 114 75
HSQC spectra recorded   18 28 18
"Good" HSQC spectra   10 14 10
HTP crystal screening   60 77 57
Crystal hit   32 40 32
X-Ray structures   7 7 7 7
NMR structures   1 1 1 1
Total structures   8 8 8 8

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[attrition table created 2015-08-22]



PSI-2 BIG domains assigned to NESG




PSI-2 BIG domains



Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   202 463 152
Expression tested   191 425 149
Expressed (E > 0)   152 298 127
Expressed-Soluble (S > 0)   125 217 102
Expressed-Soluble (ES >= 9)   110 182 88
Fermented   80 128 68
Purification attempted   80 119 68
Purified (>= 0.5 mg yield)   60 86 50
HSQC spectra recorded   54 76 46
"Good" HSQC spectra   37 49 31
HTP crystal screening   26 34 23
Crystal hit   12 14 12
X-Ray structures   4 4 4 5
NMR structures   19 19 18 202
Total structures   21 22 20 25

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[attrition table created 2015-08-22]



Human Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   86 221 62
Expression tested   75 196 59
Expressed (E > 0)   63 145 53
Expressed-Soluble (S > 0)   45 86 37
Expressed-Soluble (ES >= 9)   37 68 30
Fermented   32 53 25
Purification attempted   37 57 29
Purified (>= 0.5 mg yield)   26 41 20
HSQC spectra recorded   22 32 18
"Good" HSQC spectra   16 22 13
HTP crystal screening   9 14 7
Crystal hit   4 6 4
X-Ray structures   2 2 2 3
NMR structures   7 7 6 71
Total structures   8 9 7 10

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[attrition table created 2015-08-22]



D. Baker outreach targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   416 430 394
Expression tested   381 390 370
Expressed (E > 0)   341 345 331
Expressed-Soluble (S > 0)   333 336 323
Expressed-Soluble (ES >= 9)   307 309 298
Fermented   361 363 351
Purification attempted   315 315 306
Purified (>= 0.5 mg yield)   300 300 291
HSQC spectra recorded   137 137 131
"Good" HSQC spectra   76 76 71
HTP crystal screening   251 251 243
Crystal hit   136 136 134
X-Ray structures   52 52 51 58
NMR structures   21 21 21 23
Total structures   73 73 72 81

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[attrition table created 2015-08-22]



HCPIN (Human Cancer Interaction Networks)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   3,257 8,082 1,558
Expression tested   3,225 7,975 1,547
Expressed (E > 0)   2,710 5,956 1,444
Expressed-Soluble (S > 0)   1,758 3,325 1,090
Expressed-Soluble (ES >= 9)   1,366 2,509 888
Fermented   1,112 1,765 779
Purification attempted   957 1,459 693
Purified (>= 0.5 mg yield)   821 1,242 612
HSQC spectra recorded   392 572 304
"Good" HSQC spectra   230 300 186
HTP crystal screening   315 453 249
Crystal hit   131 153 110
X-Ray structures   29 29 28 30
NMR structures   71 71 68 726
Total structures   94 96 89 102

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[attrition table created 2015-08-22]



Mitochondrial Proteome Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   648 1,812 311
Expression tested   627 1,713 299
Expressed (E > 0)   505 1,182 262
Expressed-Soluble (S > 0)   351 654 216
Expressed-Soluble (ES >= 9)   290 523 190
Fermented   253 429 170
Purification attempted   194 319 137
Purified (>= 0.5 mg yield)   178 279 128
HSQC spectra recorded   53 86 40
"Good" HSQC spectra   21 33 14
HTP crystal screening   74 107 61
Crystal hit   19 29 18
X-Ray structures   3 6 3 6
NMR structures   4 4 3 4
Total structures   7 10 6 10

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[attrition table created 2015-08-22]



Mitochondrial Proteome Partnership - nominated targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   308 1,110 131
Expression tested   307 1,104 130
Expressed (E > 0)   260 765 122
Expressed-Soluble (S > 0)   201 430 117
Expressed-Soluble (ES >= 9)   171 337 108
Fermented   159 292 101
Purification attempted   115 205 78
Purified (>= 0.5 mg yield)   101 167 71
HSQC spectra recorded   29 53 22
"Good" HSQC spectra   10 22 7
HTP crystal screening   36 55 28
Crystal hit   9 13 8
X-Ray structures   2 2 2 2
NMR structures   4 4 3 4
Total structures   6 6 5 6

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[attrition table created 2015-08-22]



Chaperone-Enabled Studies of Epigenetic Regulation Enzymes Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   219 875 90
Expression tested   217 870 90
Expressed (E > 0)   196 749 84
Expressed-Soluble (S > 0)   128 319 70
Expressed-Soluble (ES >= 9)   99 235 59
Fermented   110 209 73
Purification attempted   84 146 62
Purified (>= 0.5 mg yield)   65 104 52
HSQC spectra recorded   19 30 16
"Good" HSQC spectra   15 22 13
HTP crystal screening   9 12 9
Crystal hit   4 4 4
X-Ray structures   1 1 1 2
NMR structures   8 8 8 8
Total structures   9 9 9 10

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2015-08-22]



Protein Homeostatis Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   205 370 90
Expression tested   183 263 79
Expressed (E > 0)   150 208 71
Expressed-Soluble (S > 0)   118 167 63
Expressed-Soluble (ES >= 9)   89 131 49
Fermented   61 77 39
Purification attempted   55 70 36
Purified (>= 0.5 mg yield)   51 64 33
HSQC spectra recorded   7 9 3
"Good" HSQC spectra   5 6 3
HTP crystal screening   24 26 18
Crystal hit   7 9 7
X-Ray structures   0 0 0 0
NMR structures   1 1 1 1
Total structures   1 1 1 1

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2015-08-21]



Human transcription factors

Production stage NESG
targets
distinct
constructs
UniProt
ids
HUGO
ids

cloned   2,809 4,355 1,793 1,784
Expressed (E > 0)   2,504 3,508 1,694 1,688
Expressed-Soluble (S > 0)   1,676 2,294 1,283 1,280
Expressed-Soluble (ES >= 9)   1,166 1,626 957 956
Purified (>= 0.5 mg yield)   1,221 1,378 1,117 1,114

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2015-08-22]



Bet v1-like super family

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   242 335 196
Expression tested   241 331 195
Expressed (E > 0)   206 276 169
Expressed-Soluble (S > 0)   168 218 149
Expressed-Soluble (ES >= 9)   141 177 129
Fermented   117 157 109
Purification attempted   109 140 103
Purified (>= 0.5 mg yield)   102 131 98
HSQC spectra recorded   79 92 78
"Good" HSQC spectra   63 74 63
HTP crystal screening   56 68 56
Crystal hit   37 40 37
X-Ray structures   18 18 18 18
NMR structures   20 20 20 232
Total structures   36 36 36 41

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2015-08-22]