NESG statistics





NESG (complete history)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   25,536 40,554 18,739
Expression tested   24,234 37,803 17,609
Expressed (E > 0)   18,764 27,189 14,161
Expressed-Soluble (S > 0)   13,880 18,452 11,349
Expressed-Soluble (ES >= 9)   11,012 14,401 9,181
Fermented   9,175 11,713 8,010
Purification attempted   8,415 10,440 7,427
Purified (>= 0.5 mg yield)   6,246 7,509 5,487
HSQC spectra recorded   2,812 3,595 2,497
"Good" HSQC spectra   1,475 1,847 1,322
HTP crystal screening   2,845 3,368 2,573
Crystal hit   1,479 1,669 1,372
X-Ray structures   607 614 596 663
NMR structures   490 492 482 52043
Total structures   1,054 1,071 1,027 1,183

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[activity table created 2014-12-19]



NESG (PSI-1)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   5,741 7,908 5,559
Expression tested   4,697 5,928 4,568
Expressed (E > 0)   3,321 3,606 3,261
Expressed-Soluble (S > 0)   2,526 2,724 2,498
Expressed-Soluble (ES >= 9)   2,026 2,150 2,009
Fermented   2,412 3,044 2,372
Purification attempted   2,154 2,593 2,120
Purified (>= 0.5 mg yield)   1,083 1,116 1,081
HSQC spectra recorded   792 1,095 789
"Good" HSQC spectra   344 484 342
HTP crystal screening   268 268 268
Crystal hit   146 146 146
X-Ray structures   112 112 112 115
NMR structures   80 80 80 832
Total structures   190 190 190 198

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[activity table created 2014-12-19]



NESG (PSI-2)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   15,302 21,621 11,016
Expression tested   14,490 20,236 10,334
Expressed (E > 0)   10,901 14,393 8,211
Expressed-Soluble (S > 0)   8,242 10,348 6,711
Expressed-Soluble (ES >= 9)   6,540 8,101 5,383
Fermented   4,214 4,999 3,602
Purification attempted   4,232 4,968 3,663
Purified (>= 0.5 mg yield)   3,213 3,771 2,754
HSQC spectra recorded   1,439 1,694 1,239
"Good" HSQC spectra   808 952 712
HTP crystal screening   1,932 2,227 1,728
Crystal hit   1,035 1,149 945
X-Ray structures   372 375 366 397
NMR structures   298 299 294 30931
Total structures   639 649 622 706

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[activity table created 2014-12-19]



NESG (PSI-Biology)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,474 13,190 4,235
Expression tested   6,157 12,584 3,994
Expressed (E > 0)   5,403 10,015 3,667
Expressed-Soluble (S > 0)   3,913 6,185 2,996
Expressed-Soluble (ES >= 9)   3,116 4,837 2,494
Fermented   3,081 4,121 2,624
Purification attempted   2,726 3,480 2,388
Purified (>= 0.5 mg yield)   2,366 2,992 2,098
HSQC spectra recorded   691 897 603
"Good" HSQC spectra   378 459 333
HTP crystal screening   736 926 679
Crystal hit   346 407 337
X-Ray structures   133 137 130 151
NMR structures   123 123 120 1286
Total structures   250 255 244 279

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[activity table created 2014-12-20]



Human proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,563 13,327 4,036
Expression tested   6,369 12,876 3,905
Expressed (E > 0)   5,204 9,549 3,326
Expressed-Soluble (S > 0)   3,416 5,543 2,462
Expressed-Soluble (ES >= 9)   2,566 4,113 1,932
Fermented   2,482 3,369 1,992
Purification attempted   2,162 2,812 1,791
Purified (>= 0.5 mg yield)   1,814 2,347 1,528
HSQC spectra recorded   519 727 411
"Good" HSQC spectra   286 368 233
HTP crystal screening   413 587 333
Crystal hit   164 195 142
X-Ray structures   42 45 41 48
NMR structures   90 90 85 918
Total structures   124 129 117 139

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[attrition table created 2014-12-20]



Eukaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   7,819 16,525 5,093
Expression tested   7,352 15,251 4,697
Expressed (E > 0)   5,888 11,089 3,854
Expressed-Soluble (S > 0)   3,911 6,432 2,861
Expressed-Soluble (ES >= 9)   2,938 4,770 2,235
Fermented   3,046 4,367 2,509
Purification attempted   2,716 3,657 2,320
Purified (>= 0.5 mg yield)   1,938 2,560 1,632
HSQC spectra recorded   710 1,091 598
"Good" HSQC spectra   351 487 295
HTP crystal screening   436 615 351
Crystal hit   172 203 150
X-Ray structures   48 51 47 57
NMR structures   116 116 111 1188
Total structures   156 161 149 175

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[attrition table created 2014-12-20]



Prokaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   17,717 24,029 13,647
Expression tested   16,882 22,553 12,913
Expressed (E > 0)   12,876 16,100 10,308
Expressed-Soluble (S > 0)   9,969 12,020 8,489
Expressed-Soluble (ES >= 9)   8,074 9,631 6,947
Fermented   6,129 7,347 5,502
Purification attempted   5,699 6,785 5,108
Purified (>= 0.5 mg yield)   4,309 4,951 3,856
HSQC spectra recorded   2,102 2,504 1,900
"Good" HSQC spectra   1,124 1,360 1,027
HTP crystal screening   2,409 2,754 2,222
Crystal hit   1,307 1,467 1,222
X-Ray structures   559 563 549 607
NMR structures   374 376 371 40235
Total structures   898 910 878 1,009

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[attrition table created 2014-12-20]



CRSH

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   176 395 142
Expression tested   176 395 142
Expressed (E > 0)   152 308 123
Expressed-Soluble (S > 0)   127 217 110
Expressed-Soluble (ES >= 9)   112 184 101
Fermented   116 167 105
Purification attempted   92 131 85
Purified (>= 0.5 mg yield)   82 114 75
HSQC spectra recorded   18 28 18
"Good" HSQC spectra   10 14 10
HTP crystal screening   60 77 57
Crystal hit   32 40 32
X-Ray structures   7 7 7 7
NMR structures   1 1 1 1
Total structures   8 8 8 8

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[attrition table created 2014-12-20]



PSI-2 BIG domains assigned to NESG




PSI-2 BIG domains



Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   200 460 150
Expression tested   191 425 149
Expressed (E > 0)   152 298 127
Expressed-Soluble (S > 0)   125 217 102
Expressed-Soluble (ES >= 9)   110 182 88
Fermented   80 128 68
Purification attempted   80 119 68
Purified (>= 0.5 mg yield)   60 86 50
HSQC spectra recorded   54 76 46
"Good" HSQC spectra   37 49 31
HTP crystal screening   26 34 23
Crystal hit   12 14 12
X-Ray structures   4 4 4 5
NMR structures   19 19 18 192
Total structures   21 22 20 24

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[attrition table created 2014-12-20]



Human Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   84 218 60
Expression tested   75 196 59
Expressed (E > 0)   63 145 53
Expressed-Soluble (S > 0)   45 86 37
Expressed-Soluble (ES >= 9)   37 68 30
Fermented   32 53 25
Purification attempted   37 57 29
Purified (>= 0.5 mg yield)   26 41 20
HSQC spectra recorded   22 32 18
"Good" HSQC spectra   16 22 13
HTP crystal screening   9 14 7
Crystal hit   4 6 4
X-Ray structures   2 2 2 3
NMR structures   7 7 6 71
Total structures   8 9 7 10

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[attrition table created 2014-12-20]



D. Baker outreach targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   416 430 394
Expression tested   381 390 370
Expressed (E > 0)   340 344 330
Expressed-Soluble (S > 0)   332 335 322
Expressed-Soluble (ES >= 9)   306 308 297
Fermented   361 363 351
Purification attempted   315 315 306
Purified (>= 0.5 mg yield)   299 299 290
HSQC spectra recorded   137 137 131
"Good" HSQC spectra   76 76 71
HTP crystal screening   251 251 243
Crystal hit   135 135 133
X-Ray structures   51 51 50 57
NMR structures   17 17 17 19
Total structures   68 68 67 76

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[attrition table created 2014-12-20]



HCPIN (Human Cancer Interaction Networks)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   3,221 8,010 1,546
Expression tested   3,194 7,929 1,535
Expressed (E > 0)   2,678 5,909 1,427
Expressed-Soluble (S > 0)   1,750 3,298 1,087
Expressed-Soluble (ES >= 9)   1,361 2,487 886
Fermented   1,075 1,707 749
Purification attempted   917 1,396 663
Purified (>= 0.5 mg yield)   786 1,186 583
HSQC spectra recorded   391 571 303
"Good" HSQC spectra   230 300 186
HTP crystal screening   315 453 249
Crystal hit   131 153 110
X-Ray structures   29 29 28 30
NMR structures   70 70 67 716
Total structures   93 95 88 101

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[attrition table created 2014-12-20]



Mitochondrial Proteome Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   647 1,799 311
Expression tested   626 1,709 299
Expressed (E > 0)   504 1,179 262
Expressed-Soluble (S > 0)   351 652 216
Expressed-Soluble (ES >= 9)   290 520 190
Fermented   252 425 170
Purification attempted   192 315 136
Purified (>= 0.5 mg yield)   176 275 127
HSQC spectra recorded   53 86 40
"Good" HSQC spectra   21 33 14
HTP crystal screening   74 107 61
Crystal hit   19 29 18
X-Ray structures   3 6 3 6
NMR structures   4 4 3 4
Total structures   7 10 6 10

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[attrition table created 2014-12-20]



Mitochondrial Proteome Partnership - nominated targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   307 1,108 131
Expression tested   306 1,103 130
Expressed (E > 0)   259 764 122
Expressed-Soluble (S > 0)   201 430 117
Expressed-Soluble (ES >= 9)   171 337 108
Fermented   158 291 101
Purification attempted   114 204 77
Purified (>= 0.5 mg yield)   100 166 70
HSQC spectra recorded   29 53 22
"Good" HSQC spectra   10 22 7
HTP crystal screening   36 55 28
Crystal hit   9 13 8
X-Ray structures   2 2 2 2
NMR structures   4 4 3 4
Total structures   6 6 5 6

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[attrition table created 2014-12-20]



Chaperone-Enabled Studies of Epigenetic Regulation Enzymes Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   210 866 86
Expression tested   208 861 86
Expressed (E > 0)   187 740 78
Expressed-Soluble (S > 0)   127 318 70
Expressed-Soluble (ES >= 9)   99 235 59
Fermented   101 200 66
Purification attempted   75 137 55
Purified (>= 0.5 mg yield)   58 97 45
HSQC spectra recorded   19 30 16
"Good" HSQC spectra   15 22 13
HTP crystal screening   9 12 9
Crystal hit   4 4 4
X-Ray structures   1 1 1 2
NMR structures   8 8 8 8
Total structures   9 9 9 10

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[attrition table created 2014-12-20]



Protein Homeostatis Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   204 359 90
Expression tested   183 262 79
Expressed (E > 0)   150 207 71
Expressed-Soluble (S > 0)   118 166 63
Expressed-Soluble (ES >= 9)   89 130 49
Fermented   61 76 39
Purification attempted   54 68 36
Purified (>= 0.5 mg yield)   50 62 33
HSQC spectra recorded   7 9 3
"Good" HSQC spectra   5 6 3
HTP crystal screening   24 26 18
Crystal hit   7 9 7
X-Ray structures   0 0 0 0
NMR structures   1 1 1 1
Total structures   1 1 1 1

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[attrition table created 2014-12-19]



Human transcription factors

Production stage NESG
targets
distinct
constructs
UniProt
ids
HUGO
ids

cloned   2,654 4,191 1,707 1,667
Expressed (E > 0)   2,334 3,332 1,593 1,559
Expressed-Soluble (S > 0)   1,635 2,248 1,253 1,244
Expressed-Soluble (ES >= 9)   1,143 1,599 937 932
Purified (>= 0.5 mg yield)   1,055 1,201 953 934

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-12-20]



Bet v1-like super family

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   242 335 196
Expression tested   241 331 195
Expressed (E > 0)   206 276 169
Expressed-Soluble (S > 0)   168 218 149
Expressed-Soluble (ES >= 9)   141 177 129
Fermented   117 157 109
Purification attempted   109 140 103
Purified (>= 0.5 mg yield)   102 131 98
HSQC spectra recorded   79 92 78
"Good" HSQC spectra   63 74 63
HTP crystal screening   56 68 56
Crystal hit   37 40 37
X-Ray structures   18 18 18 18
NMR structures   20 20 20 212
Total structures   36 36 36 39

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-12-20]