NESG statistics





NESG (complete history)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   25,488 40,464 18,716
Expression tested   24,129 37,590 17,538
Expressed (E > 0)   18,668 26,996 14,094
Expressed-Soluble (S > 0)   13,833 18,343 11,311
Expressed-Soluble (ES >= 9)   10,972 14,317 9,149
Fermented   9,092 11,619 7,937
Purification attempted   8,316 10,335 7,331
Purified (>= 0.5 mg yield)   6,144 7,400 5,388
HSQC spectra recorded   2,795 3,578 2,482
"Good" HSQC spectra   1,472 1,844 1,321
HTP crystal screening   2,843 3,366 2,571
Crystal hit   1,478 1,667 1,371
X-Ray structures   602 609 591 658
NMR structures   490 492 482 52043
Total structures   1,049 1,066 1,022 1,178

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[activity table created 2014-10-17]



NESG (PSI-1)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   5,740 7,907 5,558
Expression tested   4,698 5,929 4,568
Expressed (E > 0)   3,321 3,606 3,261
Expressed-Soluble (S > 0)   2,526 2,724 2,498
Expressed-Soluble (ES >= 9)   2,026 2,150 2,009
Fermented   2,412 3,044 2,372
Purification attempted   2,154 2,593 2,120
Purified (>= 0.5 mg yield)   1,083 1,116 1,081
HSQC spectra recorded   791 1,094 788
"Good" HSQC spectra   344 484 342
HTP crystal screening   268 268 268
Crystal hit   146 146 146
X-Ray structures   111 111 111 114
NMR structures   80 80 80 832
Total structures   189 189 189 197

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[activity table created 2014-10-17]



NESG (PSI-2)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   15,302 21,619 11,016
Expression tested   14,490 20,236 10,334
Expressed (E > 0)   10,901 14,393 8,211
Expressed-Soluble (S > 0)   8,242 10,348 6,711
Expressed-Soluble (ES >= 9)   6,540 8,101 5,383
Fermented   4,214 4,999 3,602
Purification attempted   4,232 4,968 3,663
Purified (>= 0.5 mg yield)   3,213 3,771 2,754
HSQC spectra recorded   1,439 1,694 1,239
"Good" HSQC spectra   808 952 712
HTP crystal screening   1,932 2,227 1,728
Crystal hit   1,035 1,149 945
X-Ray structures   372 375 366 397
NMR structures   298 299 294 30931
Total structures   639 649 622 706

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[activity table created 2014-10-17]



NESG (PSI-Biology)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,424 13,099 4,208
Expression tested   6,049 12,368 3,915
Expressed (E > 0)   5,304 9,819 3,594
Expressed-Soluble (S > 0)   3,864 6,073 2,956
Expressed-Soluble (ES >= 9)   3,074 4,750 2,460
Fermented   2,996 4,024 2,546
Purification attempted   2,625 3,372 2,290
Purified (>= 0.5 mg yield)   2,262 2,880 1,997
HSQC spectra recorded   674 880 588
"Good" HSQC spectra   375 456 332
HTP crystal screening   734 924 677
Crystal hit   345 405 336
X-Ray structures   129 133 126 147
NMR structures   123 123 120 1286
Total structures   246 251 240 275

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[activity table created 2014-10-18]



Human proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,519 13,247 4,017
Expression tested   6,302 12,760 3,872
Expressed (E > 0)   5,142 9,442 3,293
Expressed-Soluble (S > 0)   3,398 5,485 2,453
Expressed-Soluble (ES >= 9)   2,551 4,064 1,925
Fermented   2,423 3,303 1,941
Purification attempted   2,091 2,741 1,721
Purified (>= 0.5 mg yield)   1,732 2,263 1,447
HSQC spectra recorded   519 727 411
"Good" HSQC spectra   286 368 233
HTP crystal screening   413 587 333
Crystal hit   164 195 142
X-Ray structures   41 44 40 47
NMR structures   90 90 85 918
Total structures   123 128 116 138

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[attrition table created 2014-10-18]



Eukaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   7,775 16,440 5,074
Expression tested   7,275 15,083 4,654
Expressed (E > 0)   5,816 10,934 3,811
Expressed-Soluble (S > 0)   3,887 6,355 2,846
Expressed-Soluble (ES >= 9)   2,919 4,714 2,224
Fermented   2,987 4,301 2,458
Purification attempted   2,645 3,586 2,250
Purified (>= 0.5 mg yield)   1,856 2,476 1,551
HSQC spectra recorded   710 1,091 598
"Good" HSQC spectra   351 487 295
HTP crystal screening   436 615 351
Crystal hit   172 203 150
X-Ray structures   47 50 46 56
NMR structures   116 116 111 1188
Total structures   155 160 148 174

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[attrition table created 2014-10-18]



Prokaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   17,713 24,024 13,643
Expression tested   16,854 22,508 12,885
Expressed (E > 0)   12,852 16,062 10,284
Expressed-Soluble (S > 0)   9,946 11,988 8,466
Expressed-Soluble (ES >= 9)   8,053 9,603 6,926
Fermented   6,105 7,319 5,480
Purification attempted   5,671 6,751 5,082
Purified (>= 0.5 mg yield)   4,289 4,926 3,838
HSQC spectra recorded   2,085 2,487 1,885
"Good" HSQC spectra   1,121 1,357 1,026
HTP crystal screening   2,407 2,752 2,220
Crystal hit   1,306 1,465 1,221
X-Ray structures   555 559 545 603
NMR structures   374 376 371 40235
Total structures   894 906 874 1,005

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[attrition table created 2014-10-18]



CRSH

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   176 395 142
Expression tested   176 392 142
Expressed (E > 0)   152 305 123
Expressed-Soluble (S > 0)   127 214 110
Expressed-Soluble (ES >= 9)   112 181 101
Fermented   116 164 105
Purification attempted   92 128 85
Purified (>= 0.5 mg yield)   82 112 75
HSQC spectra recorded   18 28 18
"Good" HSQC spectra   10 14 10
HTP crystal screening   60 77 57
Crystal hit   32 39 32
X-Ray structures   6 6 6 6
NMR structures   1 1 1 1
Total structures   7 7 7 7

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[attrition table created 2014-10-18]



PSI-2 BIG domains assigned to NESG




PSI-2 BIG domains



Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   200 460 150
Expression tested   191 425 149
Expressed (E > 0)   152 298 127
Expressed-Soluble (S > 0)   125 217 102
Expressed-Soluble (ES >= 9)   110 182 88
Fermented   80 128 68
Purification attempted   80 119 68
Purified (>= 0.5 mg yield)   60 86 50
HSQC spectra recorded   54 76 46
"Good" HSQC spectra   37 49 31
HTP crystal screening   26 34 23
Crystal hit   12 14 12
X-Ray structures   4 4 4 5
NMR structures   19 19 18 192
Total structures   21 22 20 24

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[attrition table created 2014-10-18]



Human Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   84 218 60
Expression tested   75 196 59
Expressed (E > 0)   63 145 53
Expressed-Soluble (S > 0)   45 86 37
Expressed-Soluble (ES >= 9)   37 68 30
Fermented   32 53 25
Purification attempted   37 57 29
Purified (>= 0.5 mg yield)   26 41 20
HSQC spectra recorded   22 32 18
"Good" HSQC spectra   16 22 13
HTP crystal screening   9 14 7
Crystal hit   4 6 4
X-Ray structures   2 2 2 3
NMR structures   7 7 6 71
Total structures   8 9 7 10

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[attrition table created 2014-10-18]



D. Baker outreach targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   413 427 391
Expression tested   378 387 367
Expressed (E > 0)   339 343 329
Expressed-Soluble (S > 0)   331 334 321
Expressed-Soluble (ES >= 9)   305 307 296
Fermented   358 360 348
Purification attempted   312 312 303
Purified (>= 0.5 mg yield)   298 298 289
HSQC spectra recorded   135 135 129
"Good" HSQC spectra   76 76 71
HTP crystal screening   248 248 240
Crystal hit   135 135 133
X-Ray structures   50 50 49 56
NMR structures   17 17 17 19
Total structures   67 67 66 75

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[attrition table created 2014-10-18]



HCPIN (Human Cancer Interaction Networks)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   3,216 7,996 1,545
Expression tested   3,171 7,886 1,528
Expressed (E > 0)   2,657 5,868 1,420
Expressed-Soluble (S > 0)   1,738 3,271 1,082
Expressed-Soluble (ES >= 9)   1,351 2,464 882
Fermented   1,058 1,690 737
Purification attempted   899 1,378 645
Purified (>= 0.5 mg yield)   768 1,168 565
HSQC spectra recorded   391 571 303
"Good" HSQC spectra   230 300 186
HTP crystal screening   315 453 249
Crystal hit   131 153 110
X-Ray structures   29 29 28 30
NMR structures   70 70 67 716
Total structures   93 95 88 101

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[attrition table created 2014-10-18]



Mitochondrial Proteome Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   647 1,798 311
Expression tested   623 1,703 298
Expressed (E > 0)   501 1,173 261
Expressed-Soluble (S > 0)   349 650 216
Expressed-Soluble (ES >= 9)   288 518 190
Fermented   250 423 170
Purification attempted   191 314 136
Purified (>= 0.5 mg yield)   175 274 127
HSQC spectra recorded   53 86 40
"Good" HSQC spectra   21 33 14
HTP crystal screening   74 107 61
Crystal hit   19 29 18
X-Ray structures   2 5 2 5
NMR structures   4 4 3 4
Total structures   6 9 5 9

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[attrition table created 2014-10-18]



Mitochondrial Proteome Partnership - nominated targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   307 1,108 131
Expression tested   306 1,103 130
Expressed (E > 0)   259 764 122
Expressed-Soluble (S > 0)   201 430 117
Expressed-Soluble (ES >= 9)   171 337 108
Fermented   158 291 101
Purification attempted   114 204 77
Purified (>= 0.5 mg yield)   100 166 70
HSQC spectra recorded   29 53 22
"Good" HSQC spectra   10 22 7
HTP crystal screening   36 55 28
Crystal hit   9 13 8
X-Ray structures   1 1 1 1
NMR structures   4 4 3 4
Total structures   5 5 4 5

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[attrition table created 2014-10-18]



Chaperone-Enabled Studies of Epigenetic Regulation Enzymes Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   210 866 86
Expression tested   205 858 86
Expressed (E > 0)   185 738 78
Expressed-Soluble (S > 0)   127 318 70
Expressed-Soluble (ES >= 9)   99 235 59
Fermented   98 197 65
Purification attempted   73 135 53
Purified (>= 0.5 mg yield)   58 97 45
HSQC spectra recorded   19 30 16
"Good" HSQC spectra   15 22 13
HTP crystal screening   9 12 9
Crystal hit   4 4 4
X-Ray structures   1 1 1 2
NMR structures   8 8 8 8
Total structures   9 9 9 10

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-10-18]



Protein Homeostatis Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   204 359 90
Expression tested   181 260 79
Expressed (E > 0)   148 205 71
Expressed-Soluble (S > 0)   116 164 63
Expressed-Soluble (ES >= 9)   87 128 49
Fermented   59 74 39
Purification attempted   53 67 36
Purified (>= 0.5 mg yield)   49 61 33
HSQC spectra recorded   7 9 3
"Good" HSQC spectra   5 6 3
HTP crystal screening   24 26 18
Crystal hit   7 9 7
X-Ray structures   0 0 0 0
NMR structures   1 1 1 1
Total structures   1 1 1 1

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[attrition table created 2014-10-17]



Human transcription factors

Production stage NESG
targets
distinct
constructs
UniProt
ids
HUGO
ids

cloned   2,611 4,138 1,689 1,665
Expressed (E > 0)   2,280 3,272 1,561 1,557
Expressed-Soluble (S > 0)   1,626 2,233 1,246 1,244
Expressed-Soluble (ES >= 9)   1,137 1,587 932 932
Purified (>= 0.5 mg yield)   974 1,120 872 870

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-10-18]



Bet v1-like super family

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   242 335 196
Expression tested   241 331 195
Expressed (E > 0)   206 276 169
Expressed-Soluble (S > 0)   168 218 149
Expressed-Soluble (ES >= 9)   141 177 129
Fermented   117 157 109
Purification attempted   109 140 103
Purified (>= 0.5 mg yield)   102 131 98
HSQC spectra recorded   79 92 78
"Good" HSQC spectra   63 74 63
HTP crystal screening   56 68 56
Crystal hit   37 40 37
X-Ray structures   18 18 18 18
NMR structures   20 20 20 212
Total structures   36 36 36 39

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-10-18]