NESG statistics





NESG (complete history)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   25,924 41,201 19,048
Expression tested   24,479 38,088 17,773
Expressed (E > 0)   18,992 27,456 14,318
Expressed-Soluble (S > 0)   13,946 18,556 11,403
Expressed-Soluble (ES >= 9)   11,054 14,480 9,219
Fermented   9,440 12,040 8,259
Purification attempted   8,691 10,768 7,687
Purified (>= 0.5 mg yield)   6,493 7,807 5,726
HSQC spectra recorded   2,853 3,642 2,536
"Good" HSQC spectra   1,476 1,848 1,323
HTP crystal screening   2,845 3,370 2,573
Crystal hit   1,480 1,672 1,373
X-Ray structures   608 615 597 664
NMR structures   496 498 488 53543
Total structures   1,061 1,078 1,034 1,199

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[activity table created 2016-04-29]



NESG (PSI-1)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   5,790 7,957 5,603
Expression tested   4,698 5,929 4,569
Expressed (E > 0)   3,321 3,606 3,261
Expressed-Soluble (S > 0)   2,526 2,724 2,498
Expressed-Soluble (ES >= 9)   2,026 2,150 2,009
Fermented   2,412 3,044 2,372
Purification attempted   2,159 2,598 2,123
Purified (>= 0.5 mg yield)   1,088 1,121 1,086
HSQC spectra recorded   791 1,094 788
"Good" HSQC spectra   344 484 342
HTP crystal screening   268 268 268
Crystal hit   146 146 146
X-Ray structures   112 112 112 115
NMR structures   80 80 80 832
Total structures   190 190 190 198

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[activity table created 2016-04-29]



NESG (PSI-2)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   15,302 21,621 11,016
Expression tested   14,490 20,236 10,334
Expressed (E > 0)   10,901 14,393 8,211
Expressed-Soluble (S > 0)   8,242 10,348 6,711
Expressed-Soluble (ES >= 9)   6,540 8,101 5,383
Fermented   4,214 4,999 3,602
Purification attempted   4,232 4,968 3,663
Purified (>= 0.5 mg yield)   3,213 3,771 2,754
HSQC spectra recorded   1,439 1,694 1,239
"Good" HSQC spectra   808 952 712
HTP crystal screening   1,932 2,227 1,728
Crystal hit   1,035 1,149 945
X-Ray structures   372 375 366 397
NMR structures   298 299 294 30931
Total structures   639 649 622 706

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[activity table created 2016-04-30]



NESG (PSI-Biology)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,868 13,843 4,563
Expression tested   6,404 12,873 4,169
Expressed (E > 0)   5,633 10,286 3,834
Expressed-Soluble (S > 0)   3,981 6,293 3,053
Expressed-Soluble (ES >= 9)   3,160 4,919 2,536
Fermented   3,348 4,451 2,877
Purification attempted   2,997 3,804 2,648
Purified (>= 0.5 mg yield)   2,609 3,287 2,334
HSQC spectra recorded   732 944 642
"Good" HSQC spectra   379 460 334
HTP crystal screening   736 928 679
Crystal hit   347 410 338
X-Ray structures   134 138 131 152
NMR structures   134 134 131 1436
Total structures   262 267 256 295

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[activity table created 2016-04-30]



Human proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,879 13,870 4,274
Expression tested   6,544 13,073 3,999
Expressed (E > 0)   5,381 9,747 3,432
Expressed-Soluble (S > 0)   3,462 5,613 2,496
Expressed-Soluble (ES >= 9)   2,595 4,165 1,957
Fermented   2,665 3,590 2,159
Purification attempted   2,367 3,053 1,980
Purified (>= 0.5 mg yield)   1,991 2,559 1,697
HSQC spectra recorded   529 743 419
"Good" HSQC spectra   287 369 234
HTP crystal screening   413 587 333
Crystal hit   164 195 142
X-Ray structures   42 45 41 48
NMR structures   91 91 86 928
Total structures   125 130 118 140

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[attrition table created 2016-04-30]



Eukaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   8,176 17,111 5,372
Expression tested   7,567 15,493 4,831
Expressed (E > 0)   6,105 11,332 4,000
Expressed-Soluble (S > 0)   3,967 6,517 2,905
Expressed-Soluble (ES >= 9)   2,971 4,831 2,264
Fermented   3,276 4,642 2,723
Purification attempted   2,961 3,949 2,549
Purified (>= 0.5 mg yield)   2,153 2,821 1,839
HSQC spectra recorded   722 1,109 608
"Good" HSQC spectra   352 488 296
HTP crystal screening   436 615 351
Crystal hit   172 203 150
X-Ray structures   48 51 47 57
NMR structures   117 117 112 1208
Total structures   157 162 150 177

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[attrition table created 2016-04-30]



Prokaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   17,748 24,090 13,677
Expression tested   16,912 22,596 12,943
Expressed (E > 0)   12,887 16,124 10,319
Expressed-Soluble (S > 0)   9,979 12,039 8,499
Expressed-Soluble (ES >= 9)   8,083 9,649 6,956
Fermented   6,164 7,399 5,537
Purification attempted   5,730 6,821 5,139
Purified (>= 0.5 mg yield)   4,341 4,988 3,888
HSQC spectra recorded   2,131 2,533 1,929
"Good" HSQC spectra   1,124 1,360 1,027
HTP crystal screening   2,409 2,756 2,222
Crystal hit   1,308 1,470 1,223
X-Ray structures   560 564 550 608
NMR structures   379 381 376 41535
Total structures   904 916 884 1,023

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[attrition table created 2016-04-30]



CRSH

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   176 395 142
Expression tested   176 395 142
Expressed (E > 0)   152 308 123
Expressed-Soluble (S > 0)   127 217 110
Expressed-Soluble (ES >= 9)   112 184 101
Fermented   116 167 105
Purification attempted   92 131 85
Purified (>= 0.5 mg yield)   82 114 75
HSQC spectra recorded   18 28 18
"Good" HSQC spectra   10 14 10
HTP crystal screening   60 77 57
Crystal hit   32 40 32
X-Ray structures   7 7 7 7
NMR structures   1 1 1 1
Total structures   8 8 8 8

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[attrition table created 2016-04-30]



PSI-2 BIG domains assigned to NESG




PSI-2 BIG domains



Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   202 464 152
Expression tested   192 426 150
Expressed (E > 0)   153 299 128
Expressed-Soluble (S > 0)   126 218 103
Expressed-Soluble (ES >= 9)   111 183 89
Fermented   81 129 69
Purification attempted   81 120 69
Purified (>= 0.5 mg yield)   61 87 51
HSQC spectra recorded   54 76 46
"Good" HSQC spectra   37 49 31
HTP crystal screening   26 34 23
Crystal hit   12 14 12
X-Ray structures   4 4 4 5
NMR structures   19 19 18 202
Total structures   21 22 20 25

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[attrition table created 2016-04-30]



Human Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   86 222 62
Expression tested   76 197 60
Expressed (E > 0)   64 146 54
Expressed-Soluble (S > 0)   46 87 38
Expressed-Soluble (ES >= 9)   38 69 31
Fermented   33 54 26
Purification attempted   38 58 30
Purified (>= 0.5 mg yield)   27 42 21
HSQC spectra recorded   22 32 18
"Good" HSQC spectra   16 22 13
HTP crystal screening   9 14 7
Crystal hit   4 6 4
X-Ray structures   2 2 2 3
NMR structures   7 7 6 71
Total structures   8 9 7 10

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[attrition table created 2016-04-30]



D. Baker outreach targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   416 430 394
Expression tested   381 390 370
Expressed (E > 0)   341 345 331
Expressed-Soluble (S > 0)   333 336 323
Expressed-Soluble (ES >= 9)   307 309 298
Fermented   361 363 351
Purification attempted   315 315 306
Purified (>= 0.5 mg yield)   300 300 291
HSQC spectra recorded   137 137 131
"Good" HSQC spectra   76 76 71
HTP crystal screening   251 251 243
Crystal hit   136 136 134
X-Ray structures   52 52 51 58
NMR structures   21 21 21 23
Total structures   73 73 72 81

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[attrition table created 2016-04-30]



HCPIN (Human Cancer Interaction Networks)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   3,260 8,121 1,559
Expression tested   3,226 7,976 1,548
Expressed (E > 0)   2,711 5,957 1,445
Expressed-Soluble (S > 0)   1,759 3,326 1,091
Expressed-Soluble (ES >= 9)   1,367 2,511 889
Fermented   1,113 1,766 780
Purification attempted   959 1,461 695
Purified (>= 0.5 mg yield)   823 1,245 614
HSQC spectra recorded   397 579 307
"Good" HSQC spectra   231 301 187
HTP crystal screening   315 453 249
Crystal hit   131 153 110
X-Ray structures   29 29 28 30
NMR structures   71 71 68 726
Total structures   94 96 89 102

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[attrition table created 2016-04-30]



Mitochondrial Proteome Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   648 1,815 311
Expression tested   627 1,713 299
Expressed (E > 0)   505 1,182 262
Expressed-Soluble (S > 0)   351 654 216
Expressed-Soluble (ES >= 9)   290 523 190
Fermented   253 429 170
Purification attempted   194 319 137
Purified (>= 0.5 mg yield)   178 279 128
HSQC spectra recorded   57 91 44
"Good" HSQC spectra   21 33 14
HTP crystal screening   74 107 61
Crystal hit   19 29 18
X-Ray structures   3 6 3 6
NMR structures   4 4 3 4
Total structures   7 10 6 10

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[attrition table created 2016-04-30]



Mitochondrial Proteome Partnership - nominated targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   308 1,112 131
Expression tested   307 1,105 130
Expressed (E > 0)   260 766 122
Expressed-Soluble (S > 0)   201 431 117
Expressed-Soluble (ES >= 9)   171 338 108
Fermented   159 293 101
Purification attempted   115 206 78
Purified (>= 0.5 mg yield)   101 168 71
HSQC spectra recorded   29 53 22
"Good" HSQC spectra   10 22 7
HTP crystal screening   36 55 28
Crystal hit   9 13 8
X-Ray structures   2 2 2 2
NMR structures   4 4 3 4
Total structures   6 6 5 6

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[attrition table created 2016-04-30]



Chaperone-Enabled Studies of Epigenetic Regulation Enzymes Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   219 875 90
Expression tested   217 870 90
Expressed (E > 0)   196 749 84
Expressed-Soluble (S > 0)   128 319 70
Expressed-Soluble (ES >= 9)   99 235 59
Fermented   110 209 73
Purification attempted   84 146 62
Purified (>= 0.5 mg yield)   65 104 52
HSQC spectra recorded   19 30 16
"Good" HSQC spectra   15 22 13
HTP crystal screening   9 12 9
Crystal hit   4 4 4
X-Ray structures   1 1 1 2
NMR structures   8 8 8 8
Total structures   9 9 9 10

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[attrition table created 2016-04-30]



Protein Homeostatis Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   206 372 91
Expression tested   184 263 80
Expressed (E > 0)   151 208 72
Expressed-Soluble (S > 0)   119 167 64
Expressed-Soluble (ES >= 9)   90 131 50
Fermented   62 77 40
Purification attempted   56 70 37
Purified (>= 0.5 mg yield)   52 64 34
HSQC spectra recorded   10 13 6
"Good" HSQC spectra   5 6 3
HTP crystal screening   24 26 18
Crystal hit   7 9 7
X-Ray structures   0 0 0 0
NMR structures   1 1 1 1
Total structures   1 1 1 1

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[attrition table created 2016-04-29]



Human transcription factors

Production stage NESG
targets
distinct
constructs
UniProt
ids
HUGO
ids

cloned   2,849 4,395 1,833 1,784
Expressed (E > 0)   2,544 3,548 1,734 1,688
Expressed-Soluble (S > 0)   1,686 2,305 1,293 1,280
Expressed-Soluble (ES >= 9)   1,171 1,633 962 957
Purified (>= 0.5 mg yield)   1,259 1,416 1,155 1,114

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[attrition table created 2016-04-30]



Bet v1-like super family

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   242 335 196
Expression tested   241 331 195
Expressed (E > 0)   206 276 169
Expressed-Soluble (S > 0)   168 218 149
Expressed-Soluble (ES >= 9)   141 177 129
Fermented   117 157 109
Purification attempted   109 140 103
Purified (>= 0.5 mg yield)   102 131 98
HSQC spectra recorded   79 92 78
"Good" HSQC spectra   63 74 63
HTP crystal screening   56 68 56
Crystal hit   37 40 37
X-Ray structures   18 18 18 18
NMR structures   20 20 20 232
Total structures   36 36 36 41

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2016-04-30]