NESG statistics





NESG (complete history)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   25,698 40,719 18,853
Expression tested   24,305 37,882 17,650
Expressed (E > 0)   18,835 27,267 14,205
Expressed-Soluble (S > 0)   13,901 18,479 11,366
Expressed-Soluble (ES >= 9)   11,028 14,422 9,196
Fermented   9,247 11,804 8,080
Purification attempted   8,469 10,515 7,479
Purified (>= 0.5 mg yield)   6,291 7,574 5,531
HSQC spectra recorded   2,815 3,598 2,500
"Good" HSQC spectra   1,475 1,847 1,322
HTP crystal screening   2,845 3,370 2,573
Crystal hit   1,480 1,672 1,373
X-Ray structures   608 615 597 664
NMR structures   490 492 482 52043
Total structures   1,055 1,072 1,028 1,184

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[activity table created 2015-01-30]



NESG (PSI-1)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   5,740 7,907 5,558
Expression tested   4,697 5,928 4,568
Expressed (E > 0)   3,321 3,606 3,261
Expressed-Soluble (S > 0)   2,526 2,724 2,498
Expressed-Soluble (ES >= 9)   2,026 2,150 2,009
Fermented   2,412 3,044 2,372
Purification attempted   2,154 2,593 2,120
Purified (>= 0.5 mg yield)   1,083 1,116 1,081
HSQC spectra recorded   791 1,094 788
"Good" HSQC spectra   344 484 342
HTP crystal screening   268 268 268
Crystal hit   146 146 146
X-Ray structures   112 112 112 115
NMR structures   80 80 80 832
Total structures   190 190 190 198

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[activity table created 2015-01-30]



NESG (PSI-2)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   15,302 21,621 11,016
Expression tested   14,490 20,236 10,334
Expressed (E > 0)   10,901 14,393 8,211
Expressed-Soluble (S > 0)   8,242 10,348 6,711
Expressed-Soluble (ES >= 9)   6,540 8,101 5,383
Fermented   4,214 4,999 3,602
Purification attempted   4,232 4,968 3,663
Purified (>= 0.5 mg yield)   3,213 3,771 2,754
HSQC spectra recorded   1,439 1,694 1,239
"Good" HSQC spectra   808 952 712
HTP crystal screening   1,932 2,227 1,728
Crystal hit   1,035 1,149 945
X-Ray structures   372 375 366 397
NMR structures   298 299 294 30931
Total structures   639 649 622 706

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[activity table created 2015-01-30]



NESG (PSI-Biology)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,635 13,355 4,354
Expression tested   6,228 12,663 4,036
Expressed (E > 0)   5,474 10,093 3,712
Expressed-Soluble (S > 0)   3,934 6,212 3,013
Expressed-Soluble (ES >= 9)   3,132 4,858 2,510
Fermented   3,153 4,212 2,694
Purification attempted   2,780 3,555 2,441
Purified (>= 0.5 mg yield)   2,411 3,057 2,142
HSQC spectra recorded   694 900 606
"Good" HSQC spectra   378 459 333
HTP crystal screening   736 928 679
Crystal hit   347 410 338
X-Ray structures   134 138 131 152
NMR structures   123 123 120 1286
Total structures   251 256 245 280

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[activity table created 2015-01-31]



Human proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,719 13,483 4,144
Expression tested   6,435 12,946 3,941
Expressed (E > 0)   5,270 9,618 3,365
Expressed-Soluble (S > 0)   3,432 5,561 2,474
Expressed-Soluble (ES >= 9)   2,577 4,125 1,942
Fermented   2,549 3,451 2,057
Purification attempted   2,210 2,877 1,837
Purified (>= 0.5 mg yield)   1,853 2,402 1,566
HSQC spectra recorded   522 730 414
"Good" HSQC spectra   286 368 233
HTP crystal screening   413 587 333
Crystal hit   164 195 142
X-Ray structures   42 45 41 48
NMR structures   90 90 85 918
Total structures   124 129 117 139

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[attrition table created 2015-01-31]



Eukaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   7,975 16,681 5,201
Expression tested   7,418 15,325 4,733
Expressed (E > 0)   5,954 11,162 3,893
Expressed-Soluble (S > 0)   3,927 6,454 2,873
Expressed-Soluble (ES >= 9)   2,949 4,786 2,245
Fermented   3,113 4,453 2,574
Purification attempted   2,764 3,726 2,366
Purified (>= 0.5 mg yield)   1,977 2,619 1,670
HSQC spectra recorded   713 1,094 601
"Good" HSQC spectra   351 487 295
HTP crystal screening   436 615 351
Crystal hit   172 203 150
X-Ray structures   48 51 47 57
NMR structures   116 116 111 1188
Total structures   156 161 149 175

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[attrition table created 2015-01-31]



Prokaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   17,723 24,038 13,653
Expression tested   16,887 22,558 12,918
Expressed (E > 0)   12,881 16,105 10,313
Expressed-Soluble (S > 0)   9,974 12,025 8,494
Expressed-Soluble (ES >= 9)   8,079 9,636 6,952
Fermented   6,134 7,352 5,507
Purification attempted   5,705 6,791 5,114
Purified (>= 0.5 mg yield)   4,315 4,957 3,862
HSQC spectra recorded   2,102 2,504 1,900
"Good" HSQC spectra   1,124 1,360 1,027
HTP crystal screening   2,409 2,756 2,222
Crystal hit   1,308 1,470 1,223
X-Ray structures   560 564 550 608
NMR structures   374 376 371 40235
Total structures   899 911 879 1,010

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[attrition table created 2015-01-31]



CRSH

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   176 395 142
Expression tested   176 395 142
Expressed (E > 0)   152 308 123
Expressed-Soluble (S > 0)   127 217 110
Expressed-Soluble (ES >= 9)   112 184 101
Fermented   116 167 105
Purification attempted   92 131 85
Purified (>= 0.5 mg yield)   82 114 75
HSQC spectra recorded   18 28 18
"Good" HSQC spectra   10 14 10
HTP crystal screening   60 77 57
Crystal hit   32 40 32
X-Ray structures   7 7 7 7
NMR structures   1 1 1 1
Total structures   8 8 8 8

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[attrition table created 2015-01-31]



PSI-2 BIG domains assigned to NESG




PSI-2 BIG domains



Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   200 460 150
Expression tested   191 425 149
Expressed (E > 0)   152 298 127
Expressed-Soluble (S > 0)   125 217 102
Expressed-Soluble (ES >= 9)   110 182 88
Fermented   80 128 68
Purification attempted   80 119 68
Purified (>= 0.5 mg yield)   60 86 50
HSQC spectra recorded   54 76 46
"Good" HSQC spectra   37 49 31
HTP crystal screening   26 34 23
Crystal hit   12 14 12
X-Ray structures   4 4 4 5
NMR structures   19 19 18 192
Total structures   21 22 20 24

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[attrition table created 2015-01-31]



Human Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   84 218 60
Expression tested   75 196 59
Expressed (E > 0)   63 145 53
Expressed-Soluble (S > 0)   45 86 37
Expressed-Soluble (ES >= 9)   37 68 30
Fermented   32 53 25
Purification attempted   37 57 29
Purified (>= 0.5 mg yield)   26 41 20
HSQC spectra recorded   22 32 18
"Good" HSQC spectra   16 22 13
HTP crystal screening   9 14 7
Crystal hit   4 6 4
X-Ray structures   2 2 2 3
NMR structures   7 7 6 71
Total structures   8 9 7 10

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[attrition table created 2015-01-31]



D. Baker outreach targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   416 430 394
Expression tested   381 390 370
Expressed (E > 0)   340 344 330
Expressed-Soluble (S > 0)   332 335 322
Expressed-Soluble (ES >= 9)   306 308 297
Fermented   361 363 351
Purification attempted   315 315 306
Purified (>= 0.5 mg yield)   299 299 290
HSQC spectra recorded   137 137 131
"Good" HSQC spectra   76 76 71
HTP crystal screening   251 251 243
Crystal hit   136 136 134
X-Ray structures   52 52 51 58
NMR structures   17 17 17 19
Total structures   69 69 68 77

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[attrition table created 2015-01-31]



HCPIN (Human Cancer Interaction Networks)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   3,236 8,026 1,554
Expression tested   3,202 7,942 1,540
Expressed (E > 0)   2,686 5,922 1,433
Expressed-Soluble (S > 0)   1,750 3,303 1,087
Expressed-Soluble (ES >= 9)   1,362 2,492 886
Fermented   1,084 1,722 758
Purification attempted   922 1,406 667
Purified (>= 0.5 mg yield)   790 1,194 587
HSQC spectra recorded   392 572 304
"Good" HSQC spectra   230 300 186
HTP crystal screening   315 453 249
Crystal hit   131 153 110
X-Ray structures   29 29 28 30
NMR structures   70 70 67 716
Total structures   93 95 88 101

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[attrition table created 2015-01-31]



Mitochondrial Proteome Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   647 1,799 311
Expression tested   626 1,710 299
Expressed (E > 0)   504 1,179 262
Expressed-Soluble (S > 0)   351 652 216
Expressed-Soluble (ES >= 9)   290 520 190
Fermented   252 426 170
Purification attempted   192 315 136
Purified (>= 0.5 mg yield)   176 275 127
HSQC spectra recorded   53 86 40
"Good" HSQC spectra   21 33 14
HTP crystal screening   74 107 61
Crystal hit   19 29 18
X-Ray structures   3 6 3 6
NMR structures   4 4 3 4
Total structures   7 10 6 10

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[attrition table created 2015-01-31]



Mitochondrial Proteome Partnership - nominated targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   307 1,108 131
Expression tested   306 1,103 130
Expressed (E > 0)   259 764 122
Expressed-Soluble (S > 0)   201 430 117
Expressed-Soluble (ES >= 9)   171 337 108
Fermented   158 291 101
Purification attempted   114 204 77
Purified (>= 0.5 mg yield)   100 166 70
HSQC spectra recorded   29 53 22
"Good" HSQC spectra   10 22 7
HTP crystal screening   36 55 28
Crystal hit   9 13 8
X-Ray structures   2 2 2 2
NMR structures   4 4 3 4
Total structures   6 6 5 6

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[attrition table created 2015-01-31]



Chaperone-Enabled Studies of Epigenetic Regulation Enzymes Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   215 871 88
Expression tested   213 866 88
Expressed (E > 0)   192 745 81
Expressed-Soluble (S > 0)   128 319 70
Expressed-Soluble (ES >= 9)   99 235 59
Fermented   106 205 70
Purification attempted   75 137 55
Purified (>= 0.5 mg yield)   58 97 45
HSQC spectra recorded   19 30 16
"Good" HSQC spectra   15 22 13
HTP crystal screening   9 12 9
Crystal hit   4 4 4
X-Ray structures   1 1 1 2
NMR structures   8 8 8 8
Total structures   9 9 9 10

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[attrition table created 2015-01-31]



Protein Homeostatis Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   204 359 90
Expression tested   183 262 79
Expressed (E > 0)   150 207 71
Expressed-Soluble (S > 0)   118 166 63
Expressed-Soluble (ES >= 9)   89 130 49
Fermented   61 76 39
Purification attempted   54 68 36
Purified (>= 0.5 mg yield)   50 62 33
HSQC spectra recorded   7 9 3
"Good" HSQC spectra   5 6 3
HTP crystal screening   24 26 18
Crystal hit   7 9 7
X-Ray structures   0 0 0 0
NMR structures   1 1 1 1
Total structures   1 1 1 1

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[attrition table created 2015-01-30]



Human transcription factors

Production stage NESG
targets
distinct
constructs
UniProt
ids
HUGO
ids

cloned   2,757 4,294 1,765 1,667
Expressed (E > 0)   2,400 3,399 1,632 1,559
Expressed-Soluble (S > 0)   1,651 2,265 1,265 1,244
Expressed-Soluble (ES >= 9)   1,154 1,611 947 932
Purified (>= 0.5 mg yield)   1,093 1,245 990 935

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[attrition table created 2015-01-31]



Bet v1-like super family

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   242 335 196
Expression tested   241 331 195
Expressed (E > 0)   206 276 169
Expressed-Soluble (S > 0)   168 218 149
Expressed-Soluble (ES >= 9)   141 177 129
Fermented   117 157 109
Purification attempted   109 140 103
Purified (>= 0.5 mg yield)   102 131 98
HSQC spectra recorded   79 92 78
"Good" HSQC spectra   63 74 63
HTP crystal screening   56 68 56
Crystal hit   37 40 37
X-Ray structures   18 18 18 18
NMR structures   20 20 20 212
Total structures   36 36 36 39

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2015-01-31]