NESG statistics





NESG (complete history)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   25,400 40,325 18,648
Expression tested   24,042 37,497 17,497
Expressed (E > 0)   18,578 26,902 14,042
Expressed-Soluble (S > 0)   13,788 18,296 11,279
Expressed-Soluble (ES >= 9)   10,947 14,292 9,127
Fermented   8,996 11,511 7,843
Purification attempted   8,194 10,202 7,213
Purified (>= 0.5 mg yield)   6,056 7,300 5,302
HSQC spectra recorded   2,780 3,563 2,467
"Good" HSQC spectra   1,468 1,840 1,318
HTP crystal screening   2,835 3,357 2,563
Crystal hit   1,477 1,666 1,370
X-Ray structures   601 608 590 657
NMR structures   490 492 482 52043
Total structures   1,048 1,065 1,021 1,177

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[activity table created 2014-08-29]



NESG (PSI-1)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   5,740 7,907 5,558
Expression tested   4,698 5,929 4,568
Expressed (E > 0)   3,321 3,606 3,261
Expressed-Soluble (S > 0)   2,526 2,724 2,498
Expressed-Soluble (ES >= 9)   2,026 2,150 2,009
Fermented   2,412 3,044 2,372
Purification attempted   2,154 2,593 2,120
Purified (>= 0.5 mg yield)   1,083 1,116 1,081
HSQC spectra recorded   791 1,094 788
"Good" HSQC spectra   344 484 342
HTP crystal screening   268 268 268
Crystal hit   146 146 146
X-Ray structures   111 111 111 114
NMR structures   80 80 80 832
Total structures   189 189 189 197

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[activity table created 2014-08-29]



NESG (PSI-2)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   15,302 21,619 11,016
Expression tested   14,490 20,236 10,334
Expressed (E > 0)   10,901 14,393 8,211
Expressed-Soluble (S > 0)   8,242 10,348 6,711
Expressed-Soluble (ES >= 9)   6,540 8,101 5,383
Fermented   4,214 4,999 3,602
Purification attempted   4,232 4,968 3,663
Purified (>= 0.5 mg yield)   3,213 3,771 2,754
HSQC spectra recorded   1,439 1,694 1,239
"Good" HSQC spectra   808 952 712
HTP crystal screening   1,931 2,226 1,727
Crystal hit   1,035 1,149 945
X-Ray structures   372 375 366 397
NMR structures   298 299 294 30931
Total structures   639 649 622 706

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[activity table created 2014-08-29]



NESG (PSI-Biology)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,331 12,957 4,133
Expression tested   5,962 12,275 3,874
Expressed (E > 0)   5,214 9,725 3,542
Expressed-Soluble (S > 0)   3,819 6,026 2,924
Expressed-Soluble (ES >= 9)   3,049 4,725 2,438
Fermented   2,900 3,916 2,452
Purification attempted   2,502 3,239 2,172
Purified (>= 0.5 mg yield)   2,173 2,780 1,910
HSQC spectra recorded   659 865 573
"Good" HSQC spectra   371 452 329
HTP crystal screening   727 916 670
Crystal hit   344 404 335
X-Ray structures   128 132 125 146
NMR structures   123 123 120 1286
Total structures   245 250 239 274

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[activity table created 2014-08-30]



Human proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,481 13,192 3,998
Expression tested   6,234 12,688 3,848
Expressed (E > 0)   5,070 9,368 3,258
Expressed-Soluble (S > 0)   3,371 5,456 2,438
Expressed-Soluble (ES >= 9)   2,543 4,056 1,920
Fermented   2,355 3,231 1,875
Purification attempted   1,994 2,637 1,625
Purified (>= 0.5 mg yield)   1,667 2,191 1,382
HSQC spectra recorded   517 725 409
"Good" HSQC spectra   285 367 233
HTP crystal screening   412 586 332
Crystal hit   164 195 142
X-Ray structures   41 44 40 47
NMR structures   90 90 85 918
Total structures   123 128 116 138

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[attrition table created 2014-08-30]



Eukaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   7,727 16,333 5,045
Expression tested   7,207 15,009 4,630
Expressed (E > 0)   5,744 10,858 3,776
Expressed-Soluble (S > 0)   3,860 6,326 2,831
Expressed-Soluble (ES >= 9)   2,911 4,706 2,219
Fermented   2,919 4,227 2,392
Purification attempted   2,548 3,480 2,154
Purified (>= 0.5 mg yield)   1,791 2,402 1,486
HSQC spectra recorded   708 1,089 596
"Good" HSQC spectra   350 486 295
HTP crystal screening   435 614 350
Crystal hit   172 203 150
X-Ray structures   47 50 46 56
NMR structures   116 116 111 1188
Total structures   155 160 148 174

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[attrition table created 2014-08-30]



Prokaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   17,673 23,992 13,604
Expression tested   16,836 22,490 12,868
Expressed (E > 0)   12,835 16,045 10,267
Expressed-Soluble (S > 0)   9,929 11,971 8,449
Expressed-Soluble (ES >= 9)   8,036 9,586 6,909
Fermented   6,077 7,285 5,452
Purification attempted   5,646 6,724 5,060
Purified (>= 0.5 mg yield)   4,266 4,900 3,817
HSQC spectra recorded   2,072 2,474 1,872
"Good" HSQC spectra   1,118 1,354 1,023
HTP crystal screening   2,400 2,744 2,213
Crystal hit   1,305 1,464 1,220
X-Ray structures   554 558 544 602
NMR structures   374 376 371 40235
Total structures   893 905 873 1,004

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[attrition table created 2014-08-30]



CRSH

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   176 392 142
Expression tested   176 392 142
Expressed (E > 0)   152 305 123
Expressed-Soluble (S > 0)   127 214 110
Expressed-Soluble (ES >= 9)   112 181 101
Fermented   116 164 105
Purification attempted   92 128 85
Purified (>= 0.5 mg yield)   82 112 75
HSQC spectra recorded   18 28 18
"Good" HSQC spectra   10 14 10
HTP crystal screening   60 76 57
Crystal hit   32 39 32
X-Ray structures   6 6 6 6
NMR structures   1 1 1 1
Total structures   7 7 7 7

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[attrition table created 2014-08-30]



PSI-2 BIG domains assigned to NESG




PSI-2 BIG domains



Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   200 460 150
Expression tested   191 425 149
Expressed (E > 0)   152 298 127
Expressed-Soluble (S > 0)   125 217 102
Expressed-Soluble (ES >= 9)   110 182 88
Fermented   80 128 68
Purification attempted   80 118 68
Purified (>= 0.5 mg yield)   60 85 50
HSQC spectra recorded   54 76 46
"Good" HSQC spectra   37 49 31
HTP crystal screening   26 34 23
Crystal hit   12 14 12
X-Ray structures   4 4 4 5
NMR structures   19 19 18 192
Total structures   21 22 20 24

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[attrition table created 2014-08-30]



Human Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   84 218 60
Expression tested   75 196 59
Expressed (E > 0)   63 145 53
Expressed-Soluble (S > 0)   45 86 37
Expressed-Soluble (ES >= 9)   37 68 30
Fermented   32 53 25
Purification attempted   37 56 29
Purified (>= 0.5 mg yield)   26 40 20
HSQC spectra recorded   22 32 18
"Good" HSQC spectra   16 22 13
HTP crystal screening   9 14 7
Crystal hit   4 6 4
X-Ray structures   2 2 2 3
NMR structures   7 7 6 71
Total structures   8 9 7 10

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[attrition table created 2014-08-30]



D. Baker outreach targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   410 424 388
Expression tested   375 384 364
Expressed (E > 0)   337 341 327
Expressed-Soluble (S > 0)   329 332 319
Expressed-Soluble (ES >= 9)   303 305 294
Fermented   355 357 345
Purification attempted   309 309 300
Purified (>= 0.5 mg yield)   296 296 287
HSQC spectra recorded   128 128 122
"Good" HSQC spectra   73 73 68
HTP crystal screening   247 247 239
Crystal hit   134 134 132
X-Ray structures   48 48 47 54
NMR structures   17 17 17 19
Total structures   65 65 64 73

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[attrition table created 2014-08-30]



HCPIN (Human Cancer Interaction Networks)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   3,197 7,968 1,538
Expression tested   3,162 7,871 1,525
Expressed (E > 0)   2,648 5,855 1,414
Expressed-Soluble (S > 0)   1,732 3,263 1,078
Expressed-Soluble (ES >= 9)   1,350 2,463 881
Fermented   1,049 1,675 728
Purification attempted   893 1,367 639
Purified (>= 0.5 mg yield)   765 1,160 562
HSQC spectra recorded   389 569 301
"Good" HSQC spectra   230 300 186
HTP crystal screening   315 453 249
Crystal hit   131 153 110
X-Ray structures   29 29 28 30
NMR structures   70 70 67 716
Total structures   93 95 88 101

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[attrition table created 2014-08-30]



Mitochondrial Proteome Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   646 1,791 310
Expression tested   623 1,703 298
Expressed (E > 0)   501 1,173 261
Expressed-Soluble (S > 0)   349 650 216
Expressed-Soluble (ES >= 9)   288 518 190
Fermented   250 423 170
Purification attempted   191 314 136
Purified (>= 0.5 mg yield)   175 274 127
HSQC spectra recorded   53 86 40
"Good" HSQC spectra   20 32 14
HTP crystal screening   74 107 61
Crystal hit   19 29 18
X-Ray structures   2 5 2 5
NMR structures   4 4 3 4
Total structures   6 9 5 9

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[attrition table created 2014-08-30]



Mitochondrial Proteome Partnership - nominated targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   307 1,108 131
Expression tested   306 1,103 130
Expressed (E > 0)   259 764 122
Expressed-Soluble (S > 0)   201 430 117
Expressed-Soluble (ES >= 9)   171 337 108
Fermented   158 291 101
Purification attempted   114 204 77
Purified (>= 0.5 mg yield)   100 166 70
HSQC spectra recorded   29 53 22
"Good" HSQC spectra   10 22 7
HTP crystal screening   36 55 28
Crystal hit   9 13 8
X-Ray structures   1 1 1 1
NMR structures   4 4 3 4
Total structures   5 5 4 5

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[attrition table created 2014-08-30]



Chaperone-Enabled Studies of Epigenetic Regulation Enzymes Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   207 863 86
Expression tested   205 858 86
Expressed (E > 0)   185 738 78
Expressed-Soluble (S > 0)   127 318 70
Expressed-Soluble (ES >= 9)   99 235 59
Fermented   98 197 65
Purification attempted   73 135 53
Purified (>= 0.5 mg yield)   58 97 45
HSQC spectra recorded   19 30 16
"Good" HSQC spectra   15 22 13
HTP crystal screening   9 12 9
Crystal hit   4 4 4
X-Ray structures   1 1 1 2
NMR structures   8 8 8 8
Total structures   9 9 9 10

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[attrition table created 2014-08-30]



Protein Homeostatis Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   204 356 90
Expression tested   181 260 79
Expressed (E > 0)   148 205 71
Expressed-Soluble (S > 0)   116 164 63
Expressed-Soluble (ES >= 9)   87 128 49
Fermented   59 74 39
Purification attempted   53 67 36
Purified (>= 0.5 mg yield)   49 61 33
HSQC spectra recorded   7 9 3
"Good" HSQC spectra   4 5 3
HTP crystal screening   24 26 18
Crystal hit   7 9 7
X-Ray structures   0 0 0 0
NMR structures   1 1 1 1
Total structures   1 1 1 1

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[attrition table created 2014-08-29]



Human transcription factors

Production stage NESG
targets
distinct
constructs
UniProt
ids
HUGO
ids

cloned   2,581 4,102 1,672 1,664
Expressed (E > 0)   2,208 3,200 1,526 1,521
Expressed-Soluble (S > 0)   1,599 2,206 1,231 1,228
Expressed-Soluble (ES >= 9)   1,129 1,579 927 926
Purified (>= 0.5 mg yield)   910 1,052 808 807

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-08-30]



Bet v1-like super family

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   242 335 196
Expression tested   241 331 195
Expressed (E > 0)   206 276 169
Expressed-Soluble (S > 0)   168 218 149
Expressed-Soluble (ES >= 9)   141 177 129
Fermented   117 157 109
Purification attempted   109 140 103
Purified (>= 0.5 mg yield)   102 131 98
HSQC spectra recorded   79 92 78
"Good" HSQC spectra   63 74 63
HTP crystal screening   56 68 56
Crystal hit   37 40 37
X-Ray structures   18 18 18 18
NMR structures   20 20 20 212
Total structures   36 36 36 39

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-08-30]