NESG statistics





NESG (complete history)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   25,344 40,199 18,619
Expression tested   23,884 37,271 17,420
Expressed (E > 0)   18,422 26,690 13,955
Expressed-Soluble (S > 0)   13,679 18,148 11,201
Expressed-Soluble (ES >= 9)   10,866 14,185 9,062
Fermented   8,868 11,362 7,721
Purification attempted   8,132 10,127 7,157
Purified (>= 0.5 mg yield)   6,002 7,236 5,253
HSQC spectra recorded   2,780 3,563 2,467
"Good" HSQC spectra   1,468 1,840 1,318
HTP crystal screening   2,834 3,356 2,562
Crystal hit   1,471 1,660 1,365
X-Ray structures   601 608 590 657
NMR structures   489 491 481 51943
Total structures   1,047 1,064 1,020 1,176

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[activity table created 2014-07-25]



NESG (PSI-1)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   5,739 7,906 5,558
Expression tested   4,697 5,928 4,568
Expressed (E > 0)   3,321 3,606 3,261
Expressed-Soluble (S > 0)   2,526 2,724 2,498
Expressed-Soluble (ES >= 9)   2,026 2,150 2,009
Fermented   2,412 3,044 2,372
Purification attempted   2,154 2,593 2,120
Purified (>= 0.5 mg yield)   1,083 1,116 1,081
HSQC spectra recorded   791 1,094 788
"Good" HSQC spectra   344 484 342
HTP crystal screening   268 268 268
Crystal hit   146 146 146
X-Ray structures   111 111 111 114
NMR structures   80 80 80 832
Total structures   189 189 189 197

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[activity table created 2014-07-25]



NESG (PSI-2)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   15,302 21,619 11,016
Expression tested   14,490 20,236 10,334
Expressed (E > 0)   10,901 14,393 8,211
Expressed-Soluble (S > 0)   8,242 10,348 6,711
Expressed-Soluble (ES >= 9)   6,540 8,101 5,383
Fermented   4,214 4,999 3,602
Purification attempted   4,232 4,968 3,663
Purified (>= 0.5 mg yield)   3,213 3,771 2,754
HSQC spectra recorded   1,439 1,694 1,239
"Good" HSQC spectra   808 952 712
HTP crystal screening   1,931 2,226 1,727
Crystal hit   1,035 1,149 945
X-Ray structures   372 375 366 397
NMR structures   298 299 294 30931
Total structures   639 649 622 706

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[activity table created 2014-07-25]



NESG (PSI-Biology)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,275 12,831 4,104
Expression tested   5,788 12,049 3,786
Expressed (E > 0)   5,049 9,513 3,448
Expressed-Soluble (S > 0)   3,705 5,878 2,840
Expressed-Soluble (ES >= 9)   2,966 4,618 2,370
Fermented   2,771 3,767 2,330
Purification attempted   2,440 3,164 2,116
Purified (>= 0.5 mg yield)   2,119 2,716 1,861
HSQC spectra recorded   659 865 573
"Good" HSQC spectra   371 452 329
HTP crystal screening   726 915 669
Crystal hit   338 398 330
X-Ray structures   128 132 125 146
NMR structures   122 122 119 1276
Total structures   244 249 238 273

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[activity table created 2014-07-26]



Human proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,429 13,130 3,973
Expression tested   6,113 12,557 3,801
Expressed (E > 0)   4,957 9,246 3,204
Expressed-Soluble (S > 0)   3,316 5,395 2,402
Expressed-Soluble (ES >= 9)   2,519 4,027 1,903
Fermented   2,229 3,092 1,755
Purification attempted   1,936 2,574 1,573
Purified (>= 0.5 mg yield)   1,617 2,138 1,337
HSQC spectra recorded   517 725 409
"Good" HSQC spectra   285 367 233
HTP crystal screening   412 586 332
Crystal hit   162 193 140
X-Ray structures   41 44 40 47
NMR structures   90 90 85 918
Total structures   123 128 116 138

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[attrition table created 2014-07-26]



Eukaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   7,675 16,265 5,020
Expression tested   7,086 14,872 4,583
Expressed (E > 0)   5,631 10,730 3,722
Expressed-Soluble (S > 0)   3,805 6,259 2,795
Expressed-Soluble (ES >= 9)   2,887 4,671 2,202
Fermented   2,793 4,082 2,272
Purification attempted   2,490 3,411 2,102
Purified (>= 0.5 mg yield)   1,741 2,343 1,441
HSQC spectra recorded   708 1,089 596
"Good" HSQC spectra   350 486 295
HTP crystal screening   435 614 350
Crystal hit   170 201 148
X-Ray structures   47 50 46 56
NMR structures   116 116 111 1188
Total structures   155 160 148 174

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[attrition table created 2014-07-26]



Prokaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   17,669 23,934 13,600
Expression tested   16,799 22,401 12,838
Expressed (E > 0)   12,792 15,961 10,234
Expressed-Soluble (S > 0)   9,875 11,890 8,407
Expressed-Soluble (ES >= 9)   7,979 9,514 6,861
Fermented   6,075 7,281 5,450
Purification attempted   5,642 6,718 5,056
Purified (>= 0.5 mg yield)   4,262 4,895 3,813
HSQC spectra recorded   2,072 2,474 1,872
"Good" HSQC spectra   1,118 1,354 1,023
HTP crystal screening   2,399 2,743 2,212
Crystal hit   1,301 1,460 1,217
X-Ray structures   554 558 544 602
NMR structures   373 375 370 40135
Total structures   892 904 872 1,003

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[attrition table created 2014-07-26]



CRSH

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   176 392 142
Expression tested   176 391 142
Expressed (E > 0)   152 304 123
Expressed-Soluble (S > 0)   127 214 110
Expressed-Soluble (ES >= 9)   112 181 101
Fermented   116 163 105
Purification attempted   92 127 85
Purified (>= 0.5 mg yield)   82 112 75
HSQC spectra recorded   18 28 18
"Good" HSQC spectra   10 14 10
HTP crystal screening   60 76 57
Crystal hit   31 38 31
X-Ray structures   6 6 6 6
NMR structures   1 1 1 1
Total structures   7 7 7 7

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[attrition table created 2014-07-26]



PSI-2 BIG domains assigned to NESG




PSI-2 BIG domains



Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   200 458 150
Expression tested   191 424 149
Expressed (E > 0)   152 297 127
Expressed-Soluble (S > 0)   125 216 102
Expressed-Soluble (ES >= 9)   110 181 88
Fermented   80 127 68
Purification attempted   80 118 68
Purified (>= 0.5 mg yield)   60 85 50
HSQC spectra recorded   54 76 46
"Good" HSQC spectra   37 49 31
HTP crystal screening   26 34 23
Crystal hit   12 14 12
X-Ray structures   4 4 4 5
NMR structures   19 19 18 192
Total structures   21 22 20 24

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[attrition table created 2014-07-26]



Human Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   84 216 60
Expression tested   75 195 59
Expressed (E > 0)   63 144 53
Expressed-Soluble (S > 0)   45 85 37
Expressed-Soluble (ES >= 9)   37 67 30
Fermented   32 52 25
Purification attempted   37 56 29
Purified (>= 0.5 mg yield)   26 40 20
HSQC spectra recorded   22 32 18
"Good" HSQC spectra   16 22 13
HTP crystal screening   9 14 7
Crystal hit   4 6 4
X-Ray structures   2 2 2 3
NMR structures   7 7 6 71
Total structures   8 9 7 10

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[attrition table created 2014-07-26]



D. Baker outreach targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   410 424 388
Expression tested   375 384 364
Expressed (E > 0)   337 341 327
Expressed-Soluble (S > 0)   329 332 319
Expressed-Soluble (ES >= 9)   303 305 294
Fermented   355 357 345
Purification attempted   307 307 298
Purified (>= 0.5 mg yield)   294 294 285
HSQC spectra recorded   128 128 122
"Good" HSQC spectra   73 73 68
HTP crystal screening   246 246 238
Crystal hit   133 133 131
X-Ray structures   48 48 47 54
NMR structures   16 16 16 18
Total structures   64 64 63 72

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[attrition table created 2014-07-26]



HCPIN (Human Cancer Interaction Networks)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   3,197 7,954 1,538
Expression tested   3,150 7,851 1,523
Expressed (E > 0)   2,636 5,836 1,411
Expressed-Soluble (S > 0)   1,725 3,249 1,076
Expressed-Soluble (ES >= 9)   1,346 2,452 879
Fermented   1,034 1,650 718
Purification attempted   879 1,345 630
Purified (>= 0.5 mg yield)   756 1,143 557
HSQC spectra recorded   389 569 301
"Good" HSQC spectra   230 300 186
HTP crystal screening   315 453 249
Crystal hit   131 153 110
X-Ray structures   29 29 28 30
NMR structures   70 70 67 716
Total structures   93 95 88 101

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[attrition table created 2014-07-26]



Mitochondrial Proteome Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   646 1,791 310
Expression tested   623 1,703 298
Expressed (E > 0)   501 1,173 261
Expressed-Soluble (S > 0)   349 650 216
Expressed-Soluble (ES >= 9)   288 518 190
Fermented   250 423 170
Purification attempted   191 314 136
Purified (>= 0.5 mg yield)   175 274 127
HSQC spectra recorded   53 86 40
"Good" HSQC spectra   20 32 14
HTP crystal screening   74 107 61
Crystal hit   19 29 18
X-Ray structures   2 5 2 5
NMR structures   4 4 3 4
Total structures   6 9 5 9

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[attrition table created 2014-07-26]



Mitochondrial Proteome Partnership - nominated targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   307 1,108 131
Expression tested   306 1,103 130
Expressed (E > 0)   259 764 122
Expressed-Soluble (S > 0)   201 430 117
Expressed-Soluble (ES >= 9)   171 337 108
Fermented   158 291 101
Purification attempted   114 204 77
Purified (>= 0.5 mg yield)   100 166 70
HSQC spectra recorded   29 53 22
"Good" HSQC spectra   10 22 7
HTP crystal screening   36 55 28
Crystal hit   9 13 8
X-Ray structures   1 1 1 1
NMR structures   4 4 3 4
Total structures   5 5 4 5

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[attrition table created 2014-07-26]



Chaperone-Enabled Studies of Epigenetic Regulation Enzymes Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   207 863 86
Expression tested   204 857 85
Expressed (E > 0)   184 737 77
Expressed-Soluble (S > 0)   126 317 69
Expressed-Soluble (ES >= 9)   99 235 59
Fermented   97 196 64
Purification attempted   71 133 51
Purified (>= 0.5 mg yield)   56 95 43
HSQC spectra recorded   19 30 16
"Good" HSQC spectra   15 22 13
HTP crystal screening   9 12 9
Crystal hit   4 4 4
X-Ray structures   1 1 1 2
NMR structures   8 8 8 8
Total structures   9 9 9 10

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-07-26]



Protein Homeostatis Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   204 356 90
Expression tested   181 260 79
Expressed (E > 0)   148 205 71
Expressed-Soluble (S > 0)   116 164 63
Expressed-Soluble (ES >= 9)   87 128 49
Fermented   59 74 39
Purification attempted   53 67 36
Purified (>= 0.5 mg yield)   49 61 33
HSQC spectra recorded   7 9 3
"Good" HSQC spectra   4 5 3
HTP crystal screening   24 26 18
Crystal hit   7 9 7
X-Ray structures   0 0 0 0
NMR structures   1 1 1 1
Total structures   1 1 1 1

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-07-25]



Human transcription factors

Production stage NESG
targets
distinct
constructs
UniProt
ids
HUGO
ids

cloned   2,531 4,052 1,649 1,596
Expressed (E > 0)   2,097 3,083 1,474 1,470
Expressed-Soluble (S > 0)   1,546 2,148 1,197 1,195
Expressed-Soluble (ES >= 9)   1,107 1,553 912 911
Purified (>= 0.5 mg yield)   867 1,009 767 766

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-07-26]



Bet v1-like super family

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   242 335 196
Expression tested   241 331 195
Expressed (E > 0)   206 276 169
Expressed-Soluble (S > 0)   168 218 149
Expressed-Soluble (ES >= 9)   141 177 129
Fermented   117 157 109
Purification attempted   109 140 103
Purified (>= 0.5 mg yield)   102 131 98
HSQC spectra recorded   79 92 78
"Good" HSQC spectra   63 74 63
HTP crystal screening   56 68 56
Crystal hit   37 40 37
X-Ray structures   18 18 18 18
NMR structures   20 20 20 212
Total structures   36 36 36 39

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2014-07-26]