NESG statistics





NESG (complete history)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   25,700 40,722 18,854
Expression tested   24,355 37,937 17,674
Expressed (E > 0)   18,881 27,318 14,228
Expressed-Soluble (S > 0)   13,907 18,493 11,371
Expressed-Soluble (ES >= 9)   11,032 14,435 9,199
Fermented   9,298 11,864 8,130
Purification attempted   8,507 10,562 7,515
Purified (>= 0.5 mg yield)   6,324 7,616 5,564
HSQC spectra recorded   2,815 3,598 2,500
"Good" HSQC spectra   1,475 1,847 1,322
HTP crystal screening   2,845 3,370 2,573
Crystal hit   1,480 1,672 1,373
X-Ray structures   608 615 597 664
NMR structures   490 492 482 52043
Total structures   1,055 1,072 1,028 1,184

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[activity table created 2015-02-20]



NESG (PSI-1)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   5,739 7,906 5,557
Expression tested   4,697 5,928 4,568
Expressed (E > 0)   3,321 3,606 3,261
Expressed-Soluble (S > 0)   2,526 2,724 2,498
Expressed-Soluble (ES >= 9)   2,026 2,150 2,009
Fermented   2,412 3,044 2,372
Purification attempted   2,153 2,592 2,119
Purified (>= 0.5 mg yield)   1,083 1,116 1,081
HSQC spectra recorded   791 1,094 788
"Good" HSQC spectra   344 484 342
HTP crystal screening   268 268 268
Crystal hit   146 146 146
X-Ray structures   112 112 112 115
NMR structures   80 80 80 832
Total structures   190 190 190 198

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[activity table created 2015-02-20]



NESG (PSI-2)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   15,302 21,621 11,016
Expression tested   14,490 20,236 10,334
Expressed (E > 0)   10,901 14,393 8,211
Expressed-Soluble (S > 0)   8,242 10,348 6,711
Expressed-Soluble (ES >= 9)   6,540 8,101 5,383
Fermented   4,214 4,999 3,602
Purification attempted   4,232 4,968 3,663
Purified (>= 0.5 mg yield)   3,213 3,771 2,754
HSQC spectra recorded   1,439 1,694 1,239
"Good" HSQC spectra   808 952 712
HTP crystal screening   1,932 2,227 1,728
Crystal hit   1,035 1,149 945
X-Ray structures   372 375 366 397
NMR structures   298 299 294 30931
Total structures   639 649 622 706

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[activity table created 2015-02-20]



NESG (PSI-Biology)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,637 13,358 4,355
Expression tested   6,278 12,718 4,061
Expressed (E > 0)   5,520 10,144 3,736
Expressed-Soluble (S > 0)   3,940 6,226 3,018
Expressed-Soluble (ES >= 9)   3,136 4,871 2,513
Fermented   3,204 4,272 2,744
Purification attempted   2,818 3,602 2,477
Purified (>= 0.5 mg yield)   2,444 3,099 2,175
HSQC spectra recorded   694 900 606
"Good" HSQC spectra   378 459 333
HTP crystal screening   736 928 679
Crystal hit   347 410 338
X-Ray structures   134 138 131 152
NMR structures   123 123 120 1286
Total structures   251 256 245 280

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[activity table created 2015-02-21]



Human proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   6,721 13,485 4,145
Expression tested   6,485 13,000 3,965
Expressed (E > 0)   5,315 9,667 3,387
Expressed-Soluble (S > 0)   3,437 5,573 2,478
Expressed-Soluble (ES >= 9)   2,580 4,136 1,944
Fermented   2,599 3,509 2,106
Purification attempted   2,248 2,917 1,873
Purified (>= 0.5 mg yield)   1,885 2,436 1,598
HSQC spectra recorded   522 730 414
"Good" HSQC spectra   286 368 233
HTP crystal screening   413 587 333
Crystal hit   164 195 142
X-Ray structures   42 45 41 48
NMR structures   90 90 85 918
Total structures   124 129 117 139

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[attrition table created 2015-02-21]



Eukaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   7,977 16,683 5,202
Expression tested   7,468 15,380 4,757
Expressed (E > 0)   5,999 11,212 3,915
Expressed-Soluble (S > 0)   3,932 6,467 2,877
Expressed-Soluble (ES >= 9)   2,952 4,798 2,247
Fermented   3,163 4,512 2,623
Purification attempted   2,802 3,773 2,402
Purified (>= 0.5 mg yield)   2,009 2,660 1,702
HSQC spectra recorded   713 1,094 601
"Good" HSQC spectra   351 487 295
HTP crystal screening   436 615 351
Crystal hit   172 203 150
X-Ray structures   48 51 47 57
NMR structures   116 116 111 1188
Total structures   156 161 149 175

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[attrition table created 2015-02-21]



Prokaryotic proteins

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   17,723 24,039 13,653
Expression tested   16,887 22,558 12,918
Expressed (E > 0)   12,882 16,106 10,314
Expressed-Soluble (S > 0)   9,975 12,026 8,495
Expressed-Soluble (ES >= 9)   8,080 9,637 6,953
Fermented   6,135 7,353 5,508
Purification attempted   5,705 6,791 5,114
Purified (>= 0.5 mg yield)   4,316 4,958 3,863
HSQC spectra recorded   2,102 2,504 1,900
"Good" HSQC spectra   1,124 1,360 1,027
HTP crystal screening   2,409 2,756 2,222
Crystal hit   1,308 1,470 1,223
X-Ray structures   560 564 550 608
NMR structures   374 376 371 40235
Total structures   899 911 879 1,010

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[attrition table created 2015-02-21]



CRSH

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   176 395 142
Expression tested   176 395 142
Expressed (E > 0)   152 308 123
Expressed-Soluble (S > 0)   127 217 110
Expressed-Soluble (ES >= 9)   112 184 101
Fermented   116 167 105
Purification attempted   92 131 85
Purified (>= 0.5 mg yield)   82 114 75
HSQC spectra recorded   18 28 18
"Good" HSQC spectra   10 14 10
HTP crystal screening   60 77 57
Crystal hit   32 40 32
X-Ray structures   7 7 7 7
NMR structures   1 1 1 1
Total structures   8 8 8 8

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[attrition table created 2015-02-21]



PSI-2 BIG domains assigned to NESG




PSI-2 BIG domains



Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   200 460 150
Expression tested   191 425 149
Expressed (E > 0)   152 298 127
Expressed-Soluble (S > 0)   125 217 102
Expressed-Soluble (ES >= 9)   110 182 88
Fermented   80 128 68
Purification attempted   80 119 68
Purified (>= 0.5 mg yield)   60 86 50
HSQC spectra recorded   54 76 46
"Good" HSQC spectra   37 49 31
HTP crystal screening   26 34 23
Crystal hit   12 14 12
X-Ray structures   4 4 4 5
NMR structures   19 19 18 192
Total structures   21 22 20 24

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[attrition table created 2015-02-21]



Human Ubiquitin-like domains

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   84 218 60
Expression tested   75 196 59
Expressed (E > 0)   63 145 53
Expressed-Soluble (S > 0)   45 86 37
Expressed-Soluble (ES >= 9)   37 68 30
Fermented   32 53 25
Purification attempted   37 57 29
Purified (>= 0.5 mg yield)   26 41 20
HSQC spectra recorded   22 32 18
"Good" HSQC spectra   16 22 13
HTP crystal screening   9 14 7
Crystal hit   4 6 4
X-Ray structures   2 2 2 3
NMR structures   7 7 6 71
Total structures   8 9 7 10

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[attrition table created 2015-02-21]



D. Baker outreach targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   416 430 394
Expression tested   381 390 370
Expressed (E > 0)   341 345 331
Expressed-Soluble (S > 0)   333 336 323
Expressed-Soluble (ES >= 9)   307 309 298
Fermented   361 363 351
Purification attempted   315 315 306
Purified (>= 0.5 mg yield)   300 300 291
HSQC spectra recorded   137 137 131
"Good" HSQC spectra   76 76 71
HTP crystal screening   251 251 243
Crystal hit   136 136 134
X-Ray structures   52 52 51 58
NMR structures   17 17 17 19
Total structures   69 69 68 77

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[attrition table created 2015-02-21]



HCPIN (Human Cancer Interaction Networks)

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   3,236 8,026 1,554
Expression tested   3,209 7,950 1,543
Expressed (E > 0)   2,691 5,928 1,435
Expressed-Soluble (S > 0)   1,750 3,308 1,087
Expressed-Soluble (ES >= 9)   1,362 2,497 886
Fermented   1,091 1,734 765
Purification attempted   929 1,421 673
Purified (>= 0.5 mg yield)   794 1,206 591
HSQC spectra recorded   392 572 304
"Good" HSQC spectra   230 300 186
HTP crystal screening   315 453 249
Crystal hit   131 153 110
X-Ray structures   29 29 28 30
NMR structures   70 70 67 716
Total structures   93 95 88 101

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[attrition table created 2015-02-21]



Mitochondrial Proteome Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   647 1,799 311
Expression tested   626 1,710 299
Expressed (E > 0)   504 1,179 262
Expressed-Soluble (S > 0)   351 652 216
Expressed-Soluble (ES >= 9)   290 521 190
Fermented   252 426 170
Purification attempted   192 315 136
Purified (>= 0.5 mg yield)   176 275 127
HSQC spectra recorded   53 86 40
"Good" HSQC spectra   21 33 14
HTP crystal screening   74 107 61
Crystal hit   19 29 18
X-Ray structures   3 6 3 6
NMR structures   4 4 3 4
Total structures   7 10 6 10

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[attrition table created 2015-02-21]



Mitochondrial Proteome Partnership - nominated targets

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   307 1,108 131
Expression tested   306 1,103 130
Expressed (E > 0)   259 764 122
Expressed-Soluble (S > 0)   201 430 117
Expressed-Soluble (ES >= 9)   171 337 108
Fermented   158 291 101
Purification attempted   114 204 77
Purified (>= 0.5 mg yield)   100 166 70
HSQC spectra recorded   29 53 22
"Good" HSQC spectra   10 22 7
HTP crystal screening   36 55 28
Crystal hit   9 13 8
X-Ray structures   2 2 2 2
NMR structures   4 4 3 4
Total structures   6 6 5 6

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[attrition table created 2015-02-21]



Chaperone-Enabled Studies of Epigenetic Regulation Enzymes Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   215 871 88
Expression tested   213 866 88
Expressed (E > 0)   192 745 81
Expressed-Soluble (S > 0)   128 319 70
Expressed-Soluble (ES >= 9)   99 235 59
Fermented   106 205 70
Purification attempted   80 142 58
Purified (>= 0.5 mg yield)   61 100 48
HSQC spectra recorded   19 30 16
"Good" HSQC spectra   15 22 13
HTP crystal screening   9 12 9
Crystal hit   4 4 4
X-Ray structures   1 1 1 2
NMR structures   8 8 8 8
Total structures   9 9 9 10

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[attrition table created 2015-02-21]



Protein Homeostatis Partnership

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositions

cloned   204 359 90
Expression tested   183 262 79
Expressed (E > 0)   150 207 71
Expressed-Soluble (S > 0)   118 166 63
Expressed-Soluble (ES >= 9)   89 130 49
Fermented   61 76 39
Purification attempted   54 68 36
Purified (>= 0.5 mg yield)   50 62 33
HSQC spectra recorded   7 9 3
"Good" HSQC spectra   5 6 3
HTP crystal screening   24 26 18
Crystal hit   7 9 7
X-Ray structures   0 0 0 0
NMR structures   1 1 1 1
Total structures   1 1 1 1

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[attrition table created 2015-02-20]



Human transcription factors

Production stage NESG
targets
distinct
constructs
UniProt
ids
HUGO
ids

cloned   2,759 4,296 1,766 1,759
Expressed (E > 0)   2,445 3,448 1,654 1,650
Expressed-Soluble (S > 0)   1,656 2,273 1,269 1,267
Expressed-Soluble (ES >= 9)   1,157 1,617 949 949
Purified (>= 0.5 mg yield)   1,125 1,277 1,022 1,020

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2015-02-21]



Bet v1-like super family

Production stage NESG
targets
distinct
constructs
UniProt
ids
depositionsNMR & Xray

cloned   242 335 196
Expression tested   241 331 195
Expressed (E > 0)   206 276 169
Expressed-Soluble (S > 0)   168 218 149
Expressed-Soluble (ES >= 9)   141 177 129
Fermented   117 157 109
Purification attempted   109 140 103
Purified (>= 0.5 mg yield)   102 131 98
HSQC spectra recorded   79 92 78
"Good" HSQC spectra   63 74 63
HTP crystal screening   56 68 56
Crystal hit   37 40 37
X-Ray structures   18 18 18 18
NMR structures   20 20 20 212
Total structures   36 36 36 39

[top statuses]     [efficiency plots]     [weekly progress]     [topBins (targets)]
[attrition table created 2015-02-21]